Characterisation of reproductive tract microbiome and immune biomarkers for bovine genital campylobacteriosis in vaccinated and unvaccinated heifers.

Autor:	Juli MSB; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia., Raza A; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia.; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark., Forutan M; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia., Siddle HV; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia., Fordyce G; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia.; Department of Agriculture & Fisheries, Charters Towers, QLD, Australia., Muller J; Department of Agriculture & Fisheries, Charters Towers, QLD, Australia., Boe-Hansen GB; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia.; School of Veterinary Science, The University of Queensland, Gatton, QLD, Australia., Tabor AE; Centre for Animal Science, The University of Queensland, Queensland Alliance for Agriculture and Food Innovation (QAAFI), Saint Lucia, QLD, Australia.; School of Chemistry and Molecular Biosciences, The University of Queensland, Saint Lucia, QLD, Australia.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Aug 19; Vol. 15, pp. 1404525. Date of Electronic Publication: 2024 Aug 19 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1404525
Abstrakt:	Background: Bovine genital campylobacteriosis (BGC) is a globally important venereal disease of cattle caused by Campylobacter fetus subspecies venerealis . Diagnosis of BGC is highly challenging due to the lack of accurate diagnostic tests. Methods: To characterise the biomarkers for C. fetus venerealis infection, a total of twelve cycling heifers were selected and categorised as vaccinated ( n  = 6) with Vibrovax® (Zoetis™) and unvaccinated ( n  = 6). All heifers were oestrous synchronised with a double dose of prostaglandin (PGF2α) 11 days apart and when in oestrous intravaginally challenged with 2.7 x 10 9 CFU live C. fetus venerealis . DNA extracted from vaginal mucus samples was screened using a C. fetus qPCR and 16S rRNA was characterised using Illumina sequencing (V5-V8 region). Relative abundances of serum proteins were calculated using sequential window acquisition of all theoretical fragment ion spectra coupled to tandem mass spectrometry (SWATH-MS) for all heifers at three timepoints: pre-challenge, post-challenge and post-recovery. Results: In 16S rRNA sequencing of vaginal mucus, Campylobacter spp. appeared two days following challenge in unvaccinated compared to 14 days in vaccinated animals, consistent with the qPCR results. Increased relative abundances of Firmicutes and Campylobacterota were identified after C. fetus venerealis challenge and were associated with C. fetus venerealis in vaccinated and unvaccinated heifers. Greater relative abundance of Streptococcus spp. was observed during oestrous rather than dioestrous. In both vaccinated and unvaccinated heifers, Acinetobacter spp. increased after challenge with higher abundance of Corynebacterium spp. in the vaccinated group. A total of 130 unique proteins were identified in SWATH analysis of the serum samples, and the number of differentially abundant proteins found was higher in the vaccinated group after recovery from infection compared to pre-and post-challenge (adjusted P  < 0.05 and Log2FC > 0.2). Conclusion: Coglutinin, clusterin, HP homologs, vitamin D binding protein and fetuin B were identified as potential biomarkers for C. fetus venerealis infection and need further study to validate their efficiency as immune biomarkers for BGC. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Juli, Raza, Forutan, Siddle, Fordyce, Muller, Boe-Hansen and Tabor.)
Databáze:	MEDLINE
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