Rapid detection of guttae area using aniline blue staining in Fuchs endothelial corneal dystrophy mouse model.

Autor: Zhang X; Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, NHC, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, China., Qiu J; Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, NHC, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, China., Feng Y; Yixing Eye Hospital, Wuxi school of medicine, Jiangnan University, Wuxi, Jiangsu, China., Zheng J; Department of Ophthalmology, Hainan Hospital of Chinese PLA General Hospital, Sanya, China., Xiang J; Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, NHC, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, China., Gu J; Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China., Shan K; Eye Institute, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, NHC, Shanghai, China.; Key Laboratory of Myopia and Related Eye Diseases, Chinese Academy of Medical Sciences, Shanghai, China., Shi Q; Yixing Eye Hospital, Wuxi school of medicine, Jiangnan University, Wuxi, Jiangsu, China.
Jazyk: angličtina
Zdroj: Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2024 Oct; Vol. 51 (10), pp. e13921.
DOI: 10.1111/1440-1681.13921
Abstrakt: Fuchs endothelial corneal dystrophy (FECD) is a leading cause of corneal endothelial degeneration resulting in impaired visual acuity. Excessive deposition of extracellular matrix (guttae) on Descemet's membrane (DM) is the hallmark of FECD. We sought to detect the guttae area rapidly using aniline blue (AB) staining in FECD mouse model. FECD mouse model was established via ultraviolet A (UVA) exposure. Masson's trichrome staining was utilized to stain the corneal sections. AB staining was utilized to stain both whole cornea tissues and stripped Descemet's membrane-endothelium complex (DMEC) flat mounts, while immunofluorescence staining of collagen I was employed to stain guttae areas. In Masson's trichrome staining, corneal collagen fibrils were stained blue with AB. The DMEC flat mounts were stained into relative dark blue areas and relative light blue areas using 2% AB staining. The areas of dark blue could almost overlap with collagen I-positive areas, and have an acellular centre and a moderately distinct boundary line with the surrounding corneal endothelial cells. In conclusion, AB staining is a rapid and effective method for the evaluation of the guttae areas in the FECD mouse model.
(© 2024 John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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