Validation of a direct multiplex real-time reverse transcription PCR assay for rapid detection of African swine fever virus using swine field samples in Vietnam.

Autor: Shirafuji H; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan., Nishi T; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan., Kokuho T; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan., Dang HV; Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam., Truong AD; Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam., Kitamura T; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan., Watanabe M; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan., Tran HTT; Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86, Truong Chinh, Dong Da, Hanoi, Vietnam. thanhhavty@yahoo.com., Masujin K; Division of Transboundary Animal Disease Research, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo, 187-0022, Japan. masujin@affrc.go.jp.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2024 Sep 02; Vol. 17 (1), pp. 240. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1186/s13104-024-06898-2
Abstrakt: Objective: This study validates a direct multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay which was previously established for enabling rapid and simultaneous detection of African swine fever (ASF) virus (ASFV) and classical swine fever virus. The assay eliminates the need for viral nucleic acid purification using a buffer system for crude extraction and an impurity-tolerant enzyme. However, the assay had not yet been validated using field samples of ASFV-infected pigs. Therefore, to address this gap, we tested 101 samples collected from pigs in Vietnam during 2018 and 2021 for validation.
Results: The rRT-PCR assay demonstrated a diagnostic sensitivity of 98.8% and a specificity of 100%. Remarkably, crude samples yielded results comparable to those of purified samples, indicating the feasibility of using crude samples without compromising accuracy in ASFV detection. Our findings emphasize the effectiveness of the rRT-PCR assay for the prompt and accurate diagnosis of both swine fever viruses, which is essential for effective disease prevention and control in swine populations.
(© 2024. The Author(s).)
Databáze: MEDLINE
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