An outer membrane porin-lipoprotein complex modulates elongasome movement to establish cell curvature in Rhodospirillum rubrum.

Autor: Pöhl S; Department of Biology, University of Marburg, Marburg, Germany., Giacomelli G; Institute of General Microbiology, Kiel University, Kiel, Germany., Meyer FM; Institute of General Microbiology, Kiel University, Kiel, Germany., Kleeberg V; Institut für Biologie II, University of Freiburg, Freiburg, Germany.; Pädagogische Forschungsstelle Kassel, Kassel, Germany., Cohen EJ; Department of Life Sciences, Imperial College London, London, UK., Biboy J; Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK., Rosum J; Department of Biology, University of Marburg, Marburg, Germany., Glatter T; Mass Spectrometry and Proteomics Facility, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Vollmer W; Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK.; Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia., van Teeseling MCF; Department of Biology, University of Marburg, Marburg, Germany.; Institute of Microbiology, Friedrich-Schiller-Universität, Jena, Germany., Heider J; Department of Biology, University of Marburg, Marburg, Germany.; Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany., Bramkamp M; Institute of General Microbiology, Kiel University, Kiel, Germany., Thanbichler M; Department of Biology, University of Marburg, Marburg, Germany. thanbichler@uni-marburg.de.; Center for Synthetic Microbiology (SYNMIKRO), Marburg, Germany. thanbichler@uni-marburg.de.; Max Planck Fellow Group Bacterial Cell Biology, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. thanbichler@uni-marburg.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 02; Vol. 15 (1), pp. 7616. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1038/s41467-024-51790-z
Abstrakt: Curved cell shapes are widespread among bacteria and important for cellular motility, virulence and fitness. However, the underlying morphogenetic mechanisms are still incompletely understood. Here, we identify an outer-membrane protein complex that promotes cell curvature in the photosynthetic species Rhodospirillum rubrum. We show that the R. rubrum porins Por39 and Por41 form a helical ribbon-like structure at the outer curve of the cell that recruits the peptidoglycan-binding lipoprotein PapS, with PapS inactivation, porin delocalization or disruption of the porin-PapS interface resulting in cell straightening. We further demonstrate that porin-PapS assemblies act as molecular cages that entrap the cell elongation machinery, thus biasing cell growth towards the outer curve. These findings reveal a mechanistically distinct morphogenetic module mediating bacterial cell shape. Moreover, they uncover an unprecedented role of outer-membrane protein patterning in the spatial control of intracellular processes, adding an important facet to the repertoire of regulatory mechanisms in bacterial cell biology.
(© 2024. The Author(s).)
Databáze: MEDLINE
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