Lipopolysaccharide Triggers Luminal Acidification to Promote Defense Against Bacterial Infection in Vaginal Epithelium.

Autor:	Zhang YL; School of Life Sciences, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, Sun Yat-sen University, Guangzhou, China. Electronic address: zhangylin9@mail.sysu.edu.cn., Zhou YY; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Ke LJ; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Sheng J; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Zou DY; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Tang TT; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Yang ZY; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Chen L; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Hou XC; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Zhu J; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Xu JB; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China., Zhu YX; School of Life Sciences, Sun Yat-sen University, Guangzhou, China., Zhou WL; School of Life Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: lsszwl@mail.sysu.edu.cn.
Jazyk:	angličtina
Zdroj:	The American journal of pathology [Am J Pathol] 2024 Aug 31. Date of Electronic Publication: 2024 Aug 31.
DOI:	10.1016/j.ajpath.2024.08.009
Abstrakt:	The vaginal epithelium plays pivotal roles in host defense against pathogen invasion, contributing to the maintenance of an acidic microenvironment within the vaginal lumen through the activity of acid-base transport proteins. However, the precise defense mechanisms of the vaginal epithelium after a bacterial infection remain incompletely understood. This study showed that bacterial lipopolysaccharide (LPS) potentiated net proton efflux by up-regulating the expression of Na + -H + exchanger 1 (NHE1) without affecting other acid-base transport proteins in vaginal epithelial cells. Pharmacologic inhibition or genetic knockdown of Toll-like receptor-4 and the extracellular signal-regulated protein kinase signaling pathway effectively counteracted the up-regulation of NHE1 and the enhanced proton efflux triggered by LPS in vaginal epithelial cells. In vivo studies revealed that LPS administration led to luminal acidification through the up-regulation of NHE1 expression in the rat vagina. Moreover, inhibition of NHE exhibited an impaired defense against acute bacterial infection in the rat vagina. These findings collectively indicate the active involvement of vaginal epithelial cells in facilitating luminal acidification during acute bacterial infection, offering potential insights into the treatment of bacterial vaginosis. Competing Interests: Disclosure Statement None declared. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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