Overexpression of DUSP26 gene suppressed the proliferation, migration, and invasion of human prostate cancer cells.

Autor: Huang RH; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Medical College of Soochow University, Suzhou, Jiangsu, 215006, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China. Electronic address: huangruohui@gmu.edu.cn., Zeng QM; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Jiang B; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Xu G; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Xiao GC; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Xia W; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Liao YF; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Wu YT; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Zou JR; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Qian B; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Xiao RH; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Yuan YH; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Zhang GX; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China., Zou XF; Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China; Jiangxi Stone Prevention Engineering Technology Research Center, Gan Zhou, Jiang Xi, 341000, China.
Jazyk: angličtina
Zdroj: Experimental cell research [Exp Cell Res] 2024 Aug 31, pp. 114231. Date of Electronic Publication: 2024 Aug 31.
DOI: 10.1016/j.yexcr.2024.114231
Abstrakt: Prostate cancer (PCa) is threatening the health of millions of people, the pathological mechanism of prostate cancer has not been fully elaborated, and needs to be further explored. Here, we found that the expression of DUSP26 is dramatically suppressed, and a positive connection of its expression with PCa prognosis was also observed. In vitro, overexpression of DUSP26 significantly inhibited the proliferative, migrative, and invasive capacities of PC3 cells, DUSP26 silencing presented opposite results. Tumor formation experiments in subcutaneous nude mice demonstrated that DUSP26 overexpression could significantly suppress PC3 growth in vivo. Moreover, the mechanism of DUSP26 gene and PCa was discovered by RNA-Seq analysis. We found that DUSP26 significantly inhibited MAPK signaling pathway activation, and further experiments displayed that DUSP26 could impair TAK1, p38, and JNK phosphorylation. Interestingly, treatment with the TAK1 inhibitor (iTAK1) attenuated the effect of DUSP26 on PC3 cells. Together, these results suggested that DUSP26 may serve as a novel therapeutic target for PC3 cell type PCa, the underlying mechanism may be through TAK1-JNK/p38 signaling.
Competing Interests: Declaration of Competing Interest Dear Editors-in-Chief, We are pleased to submit our manuscript entitled “Dual-specificity phosphatase 26 inhibits proliferation, migration, and invasion of prostate cancer via the TAK1-JNK/p38 signaling pathway” for consideration of publication in the Cytotechnology. The manuscript has not been published or submitted for publication elsewhere. All of the authors who participated in the study agreed to the content of the manuscript and declared no conflict of interest. We would gratefully appreciate your consideration of this manuscript and thank you for the opportunity to submit this article. Sincerely, Name: Xiao-Feng Zou Address: Department of Urology, First Affiliated Hospital of Gannan Medical University, Gan Zhou, Jiang Xi, 341000, China E-mail: gyfyzouxf@126.com
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
Externí odkaz: