Zika Virus NS1 Protein Detection Using Gold Nanoparticle-Assisted Dynamic Light Scattering.

Autor: D'Amato DL; Department of Inorganic Chemistry, Universidade Federal Fluminense, Outeiro São João Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil., Bessa IAA; Department of Inorganic Chemistry, Universidade Federal Fluminense, Outeiro São João Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil., Souza ABC; Department of Inorganic Chemistry, Universidade Federal Fluminense, Outeiro São João Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil., Mendes-Monteiro L; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-170, Brazil., Mohana-Borges R; Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-170, Brazil., Allonso D; Departamento de Biotecnologia Farmacêutica, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Cidade Universitária, Rio de Janeiro, RJ, 21941-170, Brazil., Ligiero CBP; Department of Inorganic Chemistry, Universidade Federal Fluminense, Outeiro São João Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil., Ronconi CM; Department of Inorganic Chemistry, Universidade Federal Fluminense, Outeiro São João Batista s/n, Centro, Niterói, RJ, 24020-150, Brazil.
Jazyk: angličtina
Zdroj: Chemistry, an Asian journal [Chem Asian J] 2024 Sep 02, pp. e202400826. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1002/asia.202400826
Abstrakt: The Zika virus (ZIKV) is a global health threat due to its rapid spread and severe health implications, including congenital abnormalities and neurological complications. Differentiating ZIKV from other arboviruses such as dengue virus (DENV) is crucial for effective diagnosis and treatment. This study presents the development of a biosensor for detecting the ZIKV non-structural protein 1 (NS1) using gold nanoparticles (AuNPs) functionalized with monoclonal antibodies employing dynamic light scattering (DLS). The biosensor named ZINS1-mAb-AuNP exhibited specific binding to the ZIKV NS1 protein, demonstrating high colloidal stability indicated by a hydrodynamic diameter (D H ) of 140 nm, detectable via DLS. In the absence of the protein, the high ionic strength medium caused particle aggregation. This detection method showed good sensitivity and specificity, with a limit of detection (LOD) of 0.96 μg mL -1 , and avoided cross-reactivity with DENV2 NS1 and SARS-CoV-2 spike proteins. The ZINS1-mAb-AuNP biosensor represents a promising tool for the early and accurate detection of ZIKV, facilitating diagnostic and treatment capabilities for arboviral infections.
(© 2024 Wiley-VCH GmbH.)
Databáze: MEDLINE
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