The Arthropoda-specific Tramtrack group BTB protein domains use previously unknown interface to form hexamers.

Autor:	Bonchuk AN; Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russian Federation.; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russian Federation., Balagurov KI; Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russian Federation., Baradaran R; Bioscience Program, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Boyko KM; Bach Institute of Biochemistry, Research Center of Biotechnology Russian Academy of Sciences, Moscow, Russian Federation., Sluchanko NN; Bach Institute of Biochemistry, Research Center of Biotechnology Russian Academy of Sciences, Moscow, Russian Federation., Khrustaleva AM; Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russian Federation., Burtseva AD; Bach Institute of Biochemistry, Research Center of Biotechnology Russian Academy of Sciences, Moscow, Russian Federation.; Moscow Institute of Physics and Technology, Institutsky lane 9, Moscow, Russian Federation., Arkova OV; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russian Federation., Khalisova KK; Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russian Federation., Popov VO; Bach Institute of Biochemistry, Research Center of Biotechnology Russian Academy of Sciences, Moscow, Russian Federation., Naschberger A; Bioscience Program, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia., Georgiev PG; Department of the Control of Genetic Processes, Institute of Gene Biology Russian Academy of Sciences, Moscow, Russian Federation.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2024 Sep 02; Vol. 13. Date of Electronic Publication: 2024 Sep 02.
DOI:	10.7554/eLife.96832
Abstrakt:	BTB (bric-a-brack, Tramtrack, and broad complex) is a diverse group of protein-protein interaction domains found within metazoan proteins. Transcription factors contain a dimerizing BTB subtype with a characteristic N-terminal extension. The Tramtrack group (TTK) is a distinct type of BTB domain, which can multimerize. Single-particle cryo-EM microscopy revealed that the TTK-type BTB domains assemble into a hexameric structure consisting of three canonical BTB dimers connected through a previously uncharacterized interface. We demonstrated that the TTK-type BTB domains are found only in Arthropods and have undergone lineage-specific expansion in modern insects. The Drosophila genome encodes 24 transcription factors with TTK-type BTB domains, whereas only four have non-TTK-type BTB domains. Yeast two-hybrid analysis revealed that the TTK-type BTB domains have an unusually broad potential for heteromeric associations presumably through a dimer-dimer interaction interface. Thus, the TTK-type BTB domains are a structurally and functionally distinct group of protein domains specific to Arthropodan transcription factors. Competing Interests: AB, KB, RB, KB, NS, AK, AB, OA, KK, VP, AN, PG No competing interests declared (© 2024, Bonchuk et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu