Transcriptomic Analysis Reveals the Heterogeneous Role of Conducting Films Upon Electrical Stimulation.

Autor: Lawler NB; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; School of Physics, Mathematics and Computing, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Bhatt U; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Agarwal V; Cluster for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia., Evans CW; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Kaluskar P; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; Perron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.; Centre for Orthopaedic Research, The UWA Medical School, The University of Western Australia, Perth, WA, 6009, Australia.; Australian Research Council Centre for Personalised Therapeutics Technologies, University of Melbourne, Melbourne, Victoria, 3010, Australia., Amos SE; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Chen K; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; Department of Chemistry, The University of Hong Kong, Hong Kong, China., Yao Y; Electron Microscope Unit, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, 2052, Australia., Jiang H; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; Department of Chemistry, The University of Hong Kong, Hong Kong, China., Choi YS; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Zheng M; Perron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.; Centre for Orthopaedic Research, The UWA Medical School, The University of Western Australia, Perth, WA, 6009, Australia., Spagnoli D; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Suarez-Martinez I; Department of Physics and Astronomy, Curtin University, Perth, WA, 6102, Australia., Zetterlund PB; Cluster for Advanced Macromolecular Design (CAMD), School of Chemical Engineering, University of New South Wales, Sydney, NSW, 2052, Australia., Wallace VP; School of Physics, Mathematics and Computing, The University of Western Australia, Perth, WA, 6009, Australia., Harvey AR; Perron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Hodgetts SI; Perron Institute for Neurological and Translational Science, Perth, WA, 6009, Australia.; School of Human Sciences, The University of Western Australia, Perth, WA, 6009, Australia., Iyer KS; School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.; ARC Training Centre for Next-Gen Technologies in Biomedical Analysis, School of Molecular Sciences, The University of Western Australia, Perth, WA, 6009, Australia.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Sep 02, pp. e2400364. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1002/adhm.202400364
Abstrakt: Central nervous system (CNS) injuries and neurodegenerative diseases have markedly poor prognoses and can result in permanent dysfunction due to the general inability of CNS neurons to regenerate. Differentiation of transplanted stem cells has emerged as a therapeutic avenue to regenerate tissue architecture in damaged areas. Electrical stimulation is a promising approach for directing the differentiation outcomes and pattern of outgrowth of transplanted stem cells, however traditional inorganic bio-electrodes can induce adverse effects such as inflammation. This study demonstrates the implementation of two organic thin films, a polymer/reduced graphene oxide nanocomposite (P(rGO)) and PEDOT:PSS, that have favorable properties for implementation as conductive materials for electrical stimulation, as well as an inorganic indium tin oxide (ITO) conductive film. Transcriptomic analysis reveals that electrical stimulation improves neuronal differentiation of SH-SY5Y cells on all three films, with the greatest effect for P(rGO). Unique material- and electrical stimuli-mediated effects are observed, associated with differentiation, cell-substrate adhesion, and translation. The work demonstrates that P(rGO) and PEDOT:PSS are highly promising organic materials for the development of biocompatible, conductive scaffolds that will enhance electrically-aided stem cell therapeutics for CNS injuries and neurodegenerative diseases.
(© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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