Enhanced angiogenesis in porous poly(ε-caprolactone) scaffolds fortified with methacrylated hyaluronic acid hydrogel after subcutaneous transplantation.

Autor:	Yang H; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Zheng M; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Zhang Y; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Li C; Center for Pluripotent Stem Cell Research and Engineering, Research Institute of Tsinghua, Guangzhou, Guangdong Province, China., Lai JHC; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Zhang Q; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Chan KW; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China., Wang H; Center for Pluripotent Stem Cell Research and Engineering, Research Institute of Tsinghua, Guangzhou, Guangdong Province, China., Zhao X; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China., Yang Z; Center for Pluripotent Stem Cell Research and Engineering, Research Institute of Tsinghua, Guangzhou, Guangdong Province, China., Xu C; Department of Biomedical Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, China.
Jazyk:	angličtina
Zdroj:	Biomaterials translational [Biomater Transl] 2024 Mar 28; Vol. 5 (1), pp. 59-68. Date of Electronic Publication: 2024 Mar 28 (Print Publication: 2024).
DOI:	10.12336/biomatertransl.2024.01.006
Abstrakt:	A composite scaffold composed of a porous scaffold and hydrogel filling can facilitate engraftment, survival, and retention in cell transplantation processes. This study presents a composite scaffold made of poly(ε-caprolactone) (PCL) and methacrylated hyaluronic acid (MeHA) hydrogel and describes the corresponding physical properties (surface area, porosity, and mechanical strength) and host response (angiogenesis and fibrosis) after subcutaneous transplantation. Specifically, we synthesise MeHA with different degrees of substitution and fabricate a PCL scaffold with different porosities. Subsequently, we construct a series of PCL/MeHA composite scaffolds by combining these hydrogels and scaffolds. In experiments with mice, the scaffold composed of 3% PCL and 10-100 kDa, degree of substitution 70% MeHA results in the least fibrosis and a higher degree of angiogenesis. This study highlights the potential of PCL/MeHA composite scaffolds for subcutaneous cell transplantation, given their desirable physical properties and host response. Competing Interests: Conflicts of interest statement: The authors declare no conflict of interest.
Databáze:	MEDLINE
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