Comparative Evaluation of Colistin-Susceptibility Testing in Carbapenem-Resistant Klebsiella pneumoniae Using VITEK, Colistin Broth Disc Elution, and Colistin Broth Microdilution.
| Autor: | Antony T; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Senthilnathan Y; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Madhavakumar R; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Amudhan P; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Venkataraman S; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Rally S; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Pitani RS; Community Medicine, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND., Arumugam I; Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND. |
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| Jazyk: | angličtina |
| Zdroj: | Cureus [Cureus] 2024 Jul 30; Vol. 16 (7), pp. e65796. Date of Electronic Publication: 2024 Jul 30 (Print Publication: 2024). |
| DOI: | 10.7759/cureus.65796 |
| Abstrakt: | Purpose: The study aimed to compare the results of colistin-susceptibility testing performed using the automated VITEK system, colistin broth microdilution (BMD), and colistin broth disk elution (CBDE) methods. Materials and Methods: This exploratory study was conducted in a tertiary care center in South India. Carbapenem-resistant Klebsiella pneumoniae (n = 49) isolates collected from a clinical microbiology laboratory over six months (March-September 2023) were used for the study. Results: Among the 49 carbapenem-resistant Klebsiella pneumoniae isolates, 42 were found to be susceptible to carbapenem by all three methods. Seven isolates were found to be resistant to colistin using BMD and CBDE methods. Two isolates were incorrectly detected as colistin-susceptible, and one isolate was wrongly categorized as colistin-resistant using the automated VITEK system. Conclusion: CBDE is a reliable and cost-effective method that can be adopted in the routine microbiology laboratory for colistin-susceptibility testing, as it does not require any specialized equipment or techniques and is 100% consistent with the gold standard BMD method. Although the automated VITEK system is used in most routine microbiological laboratories for antibiotic-susceptibility testing, it cannot be reliably used for colistin-susceptibility testing due to its high error rates (very major error rate of 28.5%; major error rate of 2.4%). Competing Interests: Human subjects: Consent was obtained or waived by all participants in this study. Institutional Ethics Committee at Sri Ramachandra Institute of Higher Education and Research issued approval IEC-NI/22/DEC/85/129. This exploratory study was conducted in a tertiary care teaching hospital in south India, after obtaining approval from the institutional ethics committee (IEC-NI/22/DEC/85/129 dated February 21, 2023). Consent was waived for the participants. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. (Copyright © 2024, Antony et al.) |
| Databáze: | MEDLINE |
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