Temporal and spatial resolution of magnetosome degradation at the subcellular level in a 3D lung carcinoma model.

Autor: Gubieda AG; Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, 48940, Spain. alicia.gascon@ehu.eus., Gandarias L; Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, 48940, Spain.; Bioscience and Biotechnology Institute of Aix-Marseille (BIAM), Aix-Marseille Université, CNRS, CEA-UMR 7265, Saint-Paul-les-Durance, 13108, France., Pósfai M; Research Center of Biomolecular and Chemical Engineering, University of Pannonia Veszprém, Veszprém, Hungary.; HUN-REN-PE Environmental Mineralogy Research Group, Veszprém, Hungary., Pattammattel A; National Synchrotron Light Source II, Brookhaven National Laboratory, Upton, NY, 11973, USA., Fdez-Gubieda ML; Department of Electricity and Electronics, University of the Basque Country (UPV/EHU), Leioa, 48940, Spain., Abad-Díaz-de-Cerio A; Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV/EHU), Leioa, 48940, Spain. ana.abad@ehu.eus., García-Prieto A; Department of Applied Physics, University of the Basque Country (UPV/EHU), Bilbao, 48013, Spain. ana.garciap@ehu.eus.
Jazyk: angličtina
Zdroj: Journal of nanobiotechnology [J Nanobiotechnology] 2024 Sep 02; Vol. 22 (1), pp. 529. Date of Electronic Publication: 2024 Sep 02.
DOI: 10.1186/s12951-024-02788-8
Abstrakt: Magnetic nanoparticles offer many exciting possibilities in biomedicine, from cell imaging to cancer treatment. One of the currently researched nanoparticles are magnetosomes, magnetite nanoparticles of high chemical purity synthesized by magnetotactic bacteria. Despite their therapeutic potential, very little is known about their degradation in human cells, and even less so of their degradation within tumours. In an effort to explore the potential of magnetosomes for cancer treatment, we have explored their degradation process in a 3D human lung carcinoma model at the subcellular level and with nanometre scale resolution. We have used state of the art hard X-ray probes (nano-XANES and nano-XRF), which allow for identification of distinct iron phases in each region of the cell. Our results reveal the progression of magnetite oxidation to maghemite within magnetosomes, and the biosynthesis of magnetite and ferrihydrite by ferritin.
(© 2024. The Author(s).)
Databáze: MEDLINE
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