Novel comprehensive analysis of skilled reaching and grasping behavior in adult rats.

Autor: Sharma P; Division of Rehabilitation Sciences, Department of Physical Therapy, School of Health Technology and Management, USA. Electronic address: pawan.sharma@louisville.edu., Du Y; Undergraduate Biology, Stony Brook University, Stony Brook, New York 11727, USA., Singapuri K; Undergraduate Biology, Stony Brook University, Stony Brook, New York 11727, USA., Delafraz DM; Biophysics and Physiology, Stony Brook University, Stony Brook, New York 11727, USA., Shah PK; Division of Rehabilitation Sciences, Department of Physical Therapy, School of Health Technology and Management, USA.
Jazyk: angličtina
Zdroj: Journal of neuroscience methods [J Neurosci Methods] 2024 Nov; Vol. 411, pp. 110271. Date of Electronic Publication: 2024 Aug 31.
DOI: 10.1016/j.jneumeth.2024.110271
Abstrakt: Background: Reaching and grasping (R&G) in rats is commonly used as an outcome measure to investigate the effectiveness of rehabilitation or treatment strategies to recover forelimb function post spinal cord injury. Kinematic analysis has been limited to the wrist and digit movements. Kinematic profiles of the more proximal body segments that play an equally crucial role in successfully executing the task remain unexplored. Additionally, understanding of different forelimb muscle activity, their interactions, and their correlation with the kinematics of R&G movement is scarce.
New Method: In this work, novel methodologies to comprehensively assess and quantify the 3D kinematics of the proximal and distal forelimb joints along with associated muscle activity during R&G movements in adult rats are developed and discussed.
Results: Our data show that different phases of R&G identified using the novel kinematic and EMG-based approach correlate with the well-established descriptors of R&G stages derived from the Whishaw scoring system. Additionally, the developed methodology allows describing the temporal activity of individual muscles and associated mechanical and physiological properties during different phases of the motor task.
Comparison With Existing Method(s): R&G phases and their sub-components are identified and quantified using the developed kinematic and EMG-based approach. Importantly, the identified R&G phases closely match the well-established qualitative descriptors of the R&G task proposed by Whishaw and colleagues.
Conclusions: The present work provides an in-depth objective analysis of kinematics and EMG activity of R&G behavior, paving the way to a standardized approach to assessing this critical rodent motor function in future studies.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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