Development and evaluation of a TaqMan® real-time PCR assay for species-specific detection of Ehrlichia canis.

Autor: Sarli M; Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA; Ruta 34 km 227 - CP: 2300 - Rafaela, Santa Fe, Argentina., De Salvo MN; Instituto de Zoonosis Luis Pasteur; Av. Díaz Vélez 4821 - CP: 1405 - Ciudad Autónoma de Buenos Aires, Argentina., Díaz Pérez PM; Instituto de Zoonosis Luis Pasteur; Av. Díaz Vélez 4821 - CP: 1405 - Ciudad Autónoma de Buenos Aires, Argentina., Cicuttin GL; Instituto de Zoonosis Luis Pasteur; Av. Díaz Vélez 4821 - CP: 1405 - Ciudad Autónoma de Buenos Aires, Argentina., Nava S; Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA; Ruta 34 km 227 - CP: 2300 - Rafaela, Santa Fe, Argentina., Sebastian PS; Instituto de Investigación de la Cadena Láctea (IdICaL) CONICET - INTA; Ruta 34 km 227 - CP: 2300 - Rafaela, Santa Fe, Argentina. Electronic address: sebastian.patrick@inta.gob.ar.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Dec; Vol. 110 (4), pp. 116517. Date of Electronic Publication: 2024 Aug 28.
DOI: 10.1016/j.diagmicrobio.2024.116517
Abstrakt: The aim of this work was to develop a real-time PCR assay with a TaqMan® probe that detects a species-specific part of the 16S rDNA gene of Ehrlichia canis. Canine blood samples (n = 207), collected and tested by a conventional PCR assay within a study conducted by De Salvo et al., were simultaneously analyzed with the novel designed real-time PCR, and the results of both assays were compared. The agreement between the two methods was 97.6 % with a kappa value of 0.92186. Hereby, the standard error was 0.034416 and the 95 % confidence interval from 0.8544 to 0.98931. While the conventional PCR assay showed false negative results (2.42 %; 5/207), the real-time PCR assays showed a specificity of 100 %. The results of the current study showed that the developed assay presents sensitivity and specificity for the detection of E. canis in blood samples, adding a new tool for the diagnosis of this pathogen.
Competing Interests: Declaration of competing interest The authors (Macarena Sarli, María N. De Salvo, Paula M. Díaz Pérez, Gabriel L. Cicuttin, Santiago Nava, Patrick S. Sebastian) stated that there is no conflict of interest according the presented research article.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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