Unde venis? Bacterial resistance from environmental reservoirs to lettuce: tracking microbiome and resistome over a growth period.

Autor:	Gekenidis MT; Food Microbial Systems, Agroscope, 8820 Waedenswil, Switzerland., Vollenweider V; Department of Quantitative Biomedicine, University of Zurich, 8057 Zurich, Switzerland., Joyce A; Department of Biology, Maynooth University, W23 F2H6 Maynooth, Ireland., Murphy S; Department of Biology, Maynooth University, W23 F2H6 Maynooth, Ireland., Walser JC; Genetic Diversity Centre (GDC), Department of Environmental System Sciences (D-USYS), Swiss Federal Institute of Technology (ETH), 8092 Zurich, Switzerland., Ju F; Key Laboratory of Coastal Environment and Resources of Zhejiang Province, School of Engineering, Westlake University, Hangzhou, Zhejiang 310030, China.; Institute of Advanced Technology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China., Bürgmann H; Eawag, Swiss Federal Institute of Aquatic Science and Technology, 6047 Kastanienbaum, Switzerland., Hummerjohann J; Microbiological Food Safety, Agroscope, 3003 Liebefeld, Switzerland., Walsh F; Department of Biology, Maynooth University, W23 F2H6 Maynooth, Ireland., Drissner D; Department of Life Sciences, Albstadt-Sigmaringen University, 72488 Sigmaringen, Germany.
Jazyk:	angličtina
Zdroj:	FEMS microbiology ecology [FEMS Microbiol Ecol] 2024 Sep 14; Vol. 100 (10).
DOI:	10.1093/femsec/fiae118
Abstrakt:	Fresh produce is suggested to contribute highly to shaping the gut resistome. We investigated the impact of pig manure and irrigation water quality on microbiome and resistome of field-grown lettuce over an entire growth period. Lettuce was grown under four regimes, combining soil amendment with manure (with/without) with sprinkler irrigation using river water with an upstream wastewater input, disinfected by UV (with/without). Lettuce leaves, soil, and water samples were collected weekly and analysed by bacterial cultivation, 16S rRNA gene amplicon sequencing, and shotgun metagenomics from total community DNA. Cultivation yielded only few clinically relevant antibiotic-resistant bacteria (ARB), but numbers of ARB on lettuce increased over time, while no treatment-dependent changes were observed. Microbiome analysis confirmed a temporal trend. Antibiotic resistance genes (ARGs) unique to lettuce and water included multidrug and β-lactam ARGs, whereas lettuce and soil uniquely shared mainly glycopeptide and tetracycline ARGs. Surface water carried clinically relevant ARB (e.g. ESBL-producing Escherichia coli or Serratia fonticola) without affecting the overall lettuce resistome significantly. Resistance markers including biocide and metal resistance were increased in lettuce grown with manure, especially young lettuce (increased soil contact). Overall, while all investigated environments had their share as sources of the lettuce resistome, manure was the main source especially on young plants. We therefore suggest minimizing soil-vegetable contact to minimize resistance markers on fresh produce. (© The Author(s) 2024. Published by Oxford University Press on behalf of FEMS.)
Databáze:	MEDLINE
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