Unravelling the genomic landscape reveals the presence of six novel odorant-binding proteins in whitefly Bemisia tabaci Asia II-1.

Autor: Gouda MNR; Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: rudragoudamn@gmail.com., Naga KC; Division of Plant Protection, Central Potato Research Institute, Shimla, Himachal Pradesh 171001, India. Electronic address: Kailash.Naga@icar.gov.in., Nebapure SM; Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: smnebapure@gmail.com., Subramanian S; Division of Entomology, Indian Agricultural Research Institute, New Delhi 110012, India. Electronic address: entosubra@yahoo.co.in.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 1), pp. 135140. Date of Electronic Publication: 2024 Aug 30.
DOI: 10.1016/j.ijbiomac.2024.135140
Abstrakt: Genome wide analysis identified 14 OBPs in B. tabaci Asia II-1, of which six are new to science. Phylogenetic analysis traced their diversity and evolutionary lineage among Hemipteran insects. Comparative analysis reclassified the OBP gene families among B. tabaci cryptic species: Asia I, II-1, MEAM1, and MED. The 14 OBPs were clustered on four chromosomes of B. tabaci. RT-qPCR showed high expression of OBP3 and 8 across all body tissues and OBP10 in the abdomen. Molecular docking showed that OBP 3 and 10 had high affinity bonding with different candidate ligands, with binding energies ranging from -5.0 to -7.7 kcal/mol. Competitive fluorescence binding assays revealed that β-caryophyllene and limonene had high binding affinities for OBP3 and 10, with their IC50 values ranging from 9.16 to 14 μmol·L-1 and KD values around 7 to 9 μmol·L-1. Behavioural assays revealed that β-caryophyllene and carvacrol were attractants, β-ocimene and limonene were repellents, and γ-terpinene and β-ocimene were oviposition deterrents to B. tabaci. Functional validation by RNAi demonstrated that OBP3 and OBP10 modulated host recognition of B. tabaci. This study expands our understanding of the genomic landscape of OBPs in B. tabaci, offering scope for developing novel pest control strategies.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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