Assessing tissue mechanical properties: Development of a custom-made tensile device and application on rodents sciatic nerves.

Autor: Petit E; Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, J1K 2R1, QC, Canada., Bavykina V; Department of Biochemistry and Functional Genomics, Université de Sherbrooke, Sherbrooke, J1E 4K8, QC, Canada., Thibault M; Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, J1K 2R1, QC, Canada., Bilodeau A; Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, J1K 2R1, QC, Canada., Choinière W; Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, J1K 2R1, QC, Canada., Brosseau JP; Department of Biochemistry and Functional Genomics, Université de Sherbrooke, Sherbrooke, J1E 4K8, QC, Canada; Cancer Research Institute, Université de Sherbrooke, Sherbrooke, J1E 4K8, QC, Canada., Laurent B; Department of Biochemistry and Functional Genomics, Université de Sherbrooke, Sherbrooke, J1E 4K8, QC, Canada; Research Center on Aging, CIUSSS de l'ESTRIE-CHUS, Sherbrooke, J1H 4C4, QC, Canada., Lauzon MA; Department of Chemical and Biotechnological Engineering, Université de Sherbrooke, Sherbrooke, J1K 2R1, QC, Canada; Research Center on Aging, CIUSSS de l'ESTRIE-CHUS, Sherbrooke, J1H 4C4, QC, Canada; The Quebec Network for Research on Protein Function, Engineering, and Applications, Montreal, H2X 3Y7, QC, Canada. Electronic address: Marc-Antoine.Lauzon@USherbrooke.ca.
Jazyk: angličtina
Zdroj: Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2024 Nov; Vol. 159, pp. 106709. Date of Electronic Publication: 2024 Aug 26.
DOI: 10.1016/j.jmbbm.2024.106709
Abstrakt: The development of biomaterials such as synthetic scaffolds for peripheral nerve regeneration requires a precise knowledge of the mechanical properties of the nerve in physiological-like conditions. Mechanical properties (Young's modulus, maximum stress and strain at break) for peripheral nerves are scarce and large discrepancies are observed in between reports. This is due in part to the absence of a robust testing device for nerves. To overcome this limitation, a custom-made tensile device (CMTD) has been built. To evaluate its reproducibility and accuracy, the imposed speed and distance over measured speed and distance was performed, followed by a validation using poly(dimethylsiloxane) (PDMS), a commercial polymer with established mechanical properties. Finally, the mechanical characterization of rodents (mice and rats) sciatic nerves using the CMTD was performed. Mouse and rat sciatic nerves Young's modulus were 4.57 ± 2.04 and 19.2 ± 0.86 MPa respectively. Maximum stress was 1.26 ± 0.56 MPa for mice and 3.81 ± 1.84 MPa for rats. Strain at break was 53 ± 17% for mice and 32 ± 12% for rats. The number of axons per sciatic nerve was found to be twice higher for rats. Statistical analysis of the measured mechanical properties revealed no sex-related trends, for both mice and rats (except for mouse maximum stress with p=0.03). Histological evaluation of rat sciatic nerve corroborated these findings. By developing a robust CMTD to establish the key mechanical properties (Young's modulus, maximum stress and strain at break) values for rodents sciatic nerves, our work represent an essential step toward the development of better synthetic scaffolds for peripheral nerve regeneration.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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