Targeted metabolomics assisted rapid screening and characterization of aristolochic acids and their DNA adducts in aristolochia plants by ultra-high performance liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry.

Autor: Shi Y; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, PR China., Wu YW; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, PR China., Shen QX; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, PR China., Cao J; College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, PR China. Electronic address: caoj@hznu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Oct 11; Vol. 1734, pp. 465317. Date of Electronic Publication: 2024 Aug 27.
DOI: 10.1016/j.chroma.2024.465317
Abstrakt: Aristolochic acids are one of the major compounds in aristolochia plants, which are nephrotoxic and carcinogenic. A method was established for the detection and identification of aristolochic acids and their DNA adducts in four different herbs using ultra-high performance liquid chromatography-ion mobility quadrupole time-of flight mass spectrometry. Solid phase extraction conditions were optimized to improve the sensitivity of the experiment by using 40 mg of C 18 as adsorbent and 100 μL ethanol as elution solvent. At a collision energy of 10-40 eV, these compounds and cleavage patterns were precisely identified and analyzed by secondary fragmentation and collision cross section values. The obtained mass spectrometry data were then analyzed by targeted metabolomics, including principal component analysis, partial least squares-discriminant analysis and hierarchical clustering analysis, and importing the samples in the established model, the confidence values can reach 0.61 and 0.76. All in all, this method can provide a useful tool for the detection of aristolochic acids and deoxyribonucleic acid adducts. In conclusion, this method was successfully used for the detection and identification of aristolochic acids and their DNA adducts.
Competing Interests: Declaration of competing interest The authors (Ying Shi, Yi-Wen Wu, Qian-Xue Shen, Jun Cao) declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: