Protocol for detecting nitrative stress in biological lipid membranes in murine cells and tissues.
| Autor: | Aggarwal T; Department of Chemistry and Chemical Biology, Rutgers University-New Brunswick, Piscataway, NJ 08854, USA., Bellomo A; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA., Stevenson ER; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA., Herbert J; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA., Laskin DL; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA., Gow AJ; Ernest Mario School of Pharmacy, Department of Pharmacology & Toxicology, Rutgers University, New Brunswick, NJ 08901, USA., Izgu EC; Department of Chemistry and Chemical Biology, Rutgers University-New Brunswick, Piscataway, NJ 08854, USA; Cancer Institute of New Jersey, Rutgers University, New Brunswick, NJ 08901, USA; Rutgers Center for Lipid Research, New Jersey Institute for Food, Nutrition, and Health, Rutgers University-New Brunswick, New Brunswick, NJ 08901, USA. Electronic address: ec.izgu@rutgers.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Sep 20; Vol. 5 (3), pp. 103268. Date of Electronic Publication: 2024 Aug 30. |
| DOI: | 10.1016/j.xpro.2024.103268 |
| Abstrakt: | Detection of nitrative stress is crucial to understanding redox signaling and pathophysiology. Dysregulated nitrative stress, which generates high levels of peroxynitrite, can damage lipid membranes and cause activation of proinflammatory pathways associated with pulmonary complications. Here, we present a protocol for implementing a peroxynitrite-sensing phospholipid to investigate nitrative stress in murine cells and lung tissue. We detail procedures for sensing ONOO - in stimulated cells, both ex vivo and in vivo, using murine models of acute lung injury (ALI). For complete details on the use and execution of this protocol, please refer to Gutierrez and Aggarwal et al. 1 . Competing Interests: Declaration of interests E.C.I. is an inventor in a patent application filed by Rutgers University on the subject of this work. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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