Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis.

Autor:	Yoshinari A; Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Nagoya, Aichi, 464-0814 Japan., Shimizu Y; Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama, 351-0198 Japan.; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033 Japan., Hosokawa T; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531 Japan., Nakano A; Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama, 351-0198 Japan., Uemura T; Natural Science Division, Faculty of Core Research, Ochanomizu University, Tokyo, 112-8610 Japan., Takano J; Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531 Japan.; Graduate School of Agriculture, Osaka Metropolitan University, Sakai, Osaka, 599-8531 Japan.
Jazyk:	angličtina
Zdroj:	Plant & cell physiology [Plant Cell Physiol] 2024 Dec 06; Vol. 65 (11), pp. 1801-1811.
DOI:	10.1093/pcp/pcae096
Abstrakt:	Plants maintain nutrient homeostasis by controlling the activities and abundance of nutrient transporters. In Arabidopsis thaliana, the borate (B) transporter BOR1 plays a role in the efficient translocation of B under low-B conditions. BOR1 undergoes polyubiquitination in the presence of sufficient B and is then transported to the vacuole via multivesicular bodies (MVBs) to prevent B accumulation in tissues at a toxic level. A previous study indicated that BOR1 physically interacts with µ subunits of adaptor protein complexes AP-3 and AP-4, both involved in vacuolar sorting pathways. In this study, we investigated the roles of AP-3 and AP-4 subunits in BOR1 trafficking in Arabidopsis. The lack of AP-3 subunits did not affect either vacuolar sorting or polar localization of BOR1-GFP, whereas the absence of AP-4 subunits resulted in a delay in high-B-induced vacuolar sorting without affecting polar localization. Super-resolution microscopy revealed a rapid sorting of BOR1-GFP into AP-4-positive spots in the trans-Golgi network (TGN) upon high-B supply. These results indicate that AP-4 is involved in sequestration of ubiquitinated BOR1 into a TGN-specific subdomain 'vacuolar-trafficking zone', and is required for efficient sorting of MVB and vacuole. Our findings have thus helped elucidate the rapid vacuolar sorting process facilitated by AP-4 in plant nutrient transporters. (© The Author(s) 2024. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site–for further information please contact journals.permissions@oup.com.)
Databáze:	MEDLINE
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