Comparison of three rapid diagnostic tests for Plasmodium falciparum diagnosis in Ghana.

Autor: Kayode TA; Eck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA., Addo AK; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Addison TK; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Tweneboah A; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Afriyie SO; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Abbas DA; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Seth A; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA., Badu-Tawiah AK; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA., Badu K; Department of Theoretical and Applied Biology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Koepfli C; Eck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA. ckoepfli@nd.edu.
Jazyk: angličtina
Zdroj: Malaria journal [Malar J] 2024 Aug 30; Vol. 23 (1), pp. 265. Date of Electronic Publication: 2024 Aug 30.
DOI: 10.1186/s12936-024-05073-z
Abstrakt: Background: Accurate diagnosis and timely treatment are crucial in combating malaria.
Methods: A total of 449 samples were screened for Plasmodium falciparum infection by expert microscopy, qPCR, and three RDTs, namely Rapigen Biocredit Malaria Ag Pf (detecting HRP2 and pLDH on separate bands), Abbott NxTek Eliminate Malaria Ag Pf (detecting HRP2), and SD Bioline Malaria Ag Pf (detecting HRP2). hrp2/3 deletion typing was done by digital PCR.
Results: 45.7% (205/449) individuals tested positive by qPCR for P. falciparum with a mean parasite density of 12.5 parasites/μL. Using qPCR as reference, the sensitivity of microscopy was 28.3% (58/205), the Biocredit RDT was 52.2% (107/205), the NxTek RDT was 49.3% (101/205), and the Bioline RDT was 39.5% (81/205). When only samples with densities > 20 parasites/μL were included (n = 89), sensitivity of 62.9% (56/89) by microscopy, 88.8% (79/89) by Biocredit, 88.8% (79/89) by NxTek, and 78.7% (70/89) by Bioline were obtained. All three RDTs demonstrated specificities > 95%. The limits of detection (95% probability that a sample tested positive) was 4393 parasites/μL (microscopy), 56 parasites/μL (Biocredit, considering either HRP2 or pLDH), 84 parasites/μL (NxTek), and 331 parasites/μL (Bioline). None of the three qPCR-confirmed P. falciparum positive samples, identified solely through the pLDH target, or eight samples negative for all RDTs but qPCR-positive at densities > 20 parasites/µL carried hrp2/3 deletions.
Conclusion: The Biocredit and NxTek RDTs demonstrated comparable diagnostic efficacies. All three RDTs performed better than microscopy.
(© 2024. The Author(s).)
Databáze: MEDLINE
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