Effects of Bryophyllum pinnatum on Dysfunctional Autophagy in Rats Lungs Exposed to Zinc Oxide Nanoparticles.

Autor: Ijatuyi TT; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria., Lawal AO; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria; Precision Molecular Laboratory, Akure, Ondo State, Nigeria., Akinjiyan MO; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria; Medical Biochemistry Department, School of Basic Medical Sciences, Federal University of Technology, Akure, Ondo State, Nigeria. Electronic address: moakinjiyan@futa.edu.ng., Ojo FM; Medical Biochemistry Department, School of Basic Medical Sciences, Federal University of Technology, Akure, Ondo State, Nigeria., Koledoye OF; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria., Agboola OO; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria., Dahunsi DT; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria., Folorunso IM; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria; Precision Molecular Laboratory, Akure, Ondo State, Nigeria., Elekofehinti OO; Bioinformatics and Molecular Biology Unit, Department of Biochemistry, School of Life Sciences, Federal University of Technology, Akure, P.M.B. 704, Akure, Ondo-State, Nigeria.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2024 Nov 15; Vol. 141, pp. 113005. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1016/j.intimp.2024.113005
Abstrakt: Lung inflammation as a result of exposure to toxicants is a major pathological problem. Autophagy (AP) is a process of cell self-digestion and can be disrupted by environmental toxicants, leading to oxidative stress, inflammation and cellular damage. Bryophyllum pinnatum (Lam.) Oken has been used in folklore medicine to manage pathological abnormalities, including inflammation, but mechanisms remain unclear. This work investigated the effects of Bryophyllum pinnatum ethanol leaf extract (BP) on dysfunctional AP in the lungs of Wistar rats exposed to zinc oxide nanoparticles (ZONPs). The experimental rats were orally administered ZONPs for seven days (10 mg/kg). Some exposed rats were post-treated with BP (62.5 and 125 mg/kg) through oral gavage. Oxidative stress, inflammation, and apoptotic and autophagic parameters were assessed using biochemical assay and gene expression methods. Several indices of pulmonary damage were also evaluated. PCR analysis suggested that ZONP downregulated the expression of pro-autophagy-related genes (Beclin 2, ATG5, DAPK, and FOXP3) and upregulated the expression of the TNF-alpha, NF-Kb, LC3 and Bcl2 genes. In contrast, BP significantly (p < 0.0001) reversed ZONP-induced pulmonary toxicity and oxidative stress. It reduced MDA levels and increased SOD, CAT, GSH and GPxD activities. BP significantly (p < 0.0001) downregulated the expressions of proinflammatory genes (IL-6 and JNK) and upregulated the expressions of IL-10, CAT and SOD genes in ZONP-exposed rats. BP restored the lung's histoarchitectural structure after ZNOP-induced distortion. The results suggested that BP has antioxidant and anti-inflammatory properties, and could effectively restore ZNOP-induced dysfunctional AP in the lungs of Wistar rats.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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