Mastocytosis and intraepithelial lymphocytosis in the ileum and colon characterize chronic Toxoplasma gondii infection in mice.

Autor: Aguiar A; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Menezes de Brito ASS; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Santos AGAD; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Watanabe PDS; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Cuman RKN; Graduate Program in Pharmaceutical Sciences, State University of Maringá, Maringá, Paraná, Brazil., Trevizan AR; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., de Lima LL; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Bersani-Amado CA; Graduate Program in Pharmaceutical Sciences, State University of Maringá, Maringá, Paraná, Brazil., Rinaldi JC; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Sant Ana DMG; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil., Nogueira-Melo GA; Graduate Program in Biosciences and Pathophysiology, State University of Maringá, Maringá, Paraná, Brazil. Electronic address: ganmelo2@uem.br.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2024 Aug 23; Vol. 91, pp. 102533. Date of Electronic Publication: 2024 Aug 23.
DOI: 10.1016/j.tice.2024.102533
Abstrakt: Toxoplasma gondii is the causative agent of toxoplasmosis, a common zoonotic disease affecting vertebrates with high global incidence. For the parasite to disseminate throughout the body, it crosses the intestinal barrier, triggering inflammatory reactions. This study aimed to assess the tissue response in the ileum and colon of mice following chronic infection with T. gondii. Fourteen mice were divided into two groups: the infected group received 1000 T. gondii oocysts via gavage, and after 60 days, the mice were euthanized. The ileum and colon were collected and processed for histological analysis, inflammatory marker measurement and myenteric neuron analysis. Chronic infection resulted in a significant increase in intraepithelial lymphocytes and mast cells, as well as morphometric changes such as increased total intestinal wall thickness of the ileum, crypt depth, collagen fiber area, and a decrease in myeloperoxidase activity, without altering nitric oxide levels. While the number of myenteric neurons remained unchanged, there was an increase in vasoactive intestinal peptide expression. These results suggest persistence intestinal inflammatory stimuli in chronic T. gondii infection.
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Databáze: MEDLINE
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