Unravelling the antioxidant behaviour of self-assembly β-Sheet in silk fibroin.

Autor:	Qian Z; Department of Anatomy the Basic Medicine College, Inner Mongolia Medical University, Hohhot, 010000, Inner Mongolia, China; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Sun C; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Li Q; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Xie Y; Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China., Zhan L; Institute for Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Liu X; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Wang G; Institute for Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China., Wei Y; The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing, 100084, China; School of Materials Science and Engineering, North Minzu University, Yinchuan, 750021, China. Electronic address: weiyen@tsinghua.edu.cn., Qiu J; Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China. Electronic address: jhqiu@cqu.edu.cn., Peng Q; Institute of Systems and Physical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China. Electronic address: pengqin@szbl.ac.cn.
Jazyk:	angličtina
Zdroj:	Redox biology [Redox Biol] 2024 Oct; Vol. 76, pp. 103307. Date of Electronic Publication: 2024 Aug 20.
DOI:	10.1016/j.redox.2024.103307
Abstrakt:	Local oxidative stress in diseases or injury severely hinders cell homeostasis and organ regeneration. Antioxidant therapy is an effective strategy for oxidative stress treatment. Biomaterials with good biocompatibility and reactive oxygen species (ROS) scavenging ability are good choices for antioxidant therapeutics. However, there are few natural biomaterials that are identified with both biocompatibility and strong antioxidant activity. Here, we show, for the first time, that silk fibroin (SF) is a strong antioxidant, which can eliminate ROS in both cells and zebrafish. We further demonstrate that the β-sheet structures turn into a random coiled structure when SF is treated with hydrogen peroxide. The content of β-sheet structures can be increased by heating, thus enhancing the antioxidation properties of SF. Therefore, SF can serve as a good antioxidant biomaterial for therapeutics, and its β-sheet structure-based antioxidation mechanism provides a novel theoretical basis, which could be a new cue for more antioxidant biomaterial discovery and identification. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024. Published by Elsevier B.V.)
Databáze:	MEDLINE
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