pyMS-Vis, an Open-Source Python Application for Visualizing and Investigating Deconvoluted Top-Down Mass Spectrometric Experiments: A Histone Proteoform Case Study.

Autor:	Pesavento JJ; Saint Mary's College of California, Moraga, California 94575, United States., Bindra MS; Saint Mary's College of California, Moraga, California 94575, United States., Das U; Saint Mary's College of California, Moraga, California 94575, United States., Rommelfanger SR; Donald Danforth Plant Science Center, St. Louis, Missouri 63132, United States.; Washington University in St. Louis, St. Louis, Missouri 63130, United States., Zhou M; Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States., Paša-Tolić L; Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99354, United States., Umen JG; Donald Danforth Plant Science Center, St. Louis, Missouri 63132, United States.; Washington University in St. Louis, St. Louis, Missouri 63130, United States.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2024 Sep 17; Vol. 96 (37), pp. 14727-14733. Date of Electronic Publication: 2024 Aug 30.
DOI:	10.1021/acs.analchem.4c02650
Abstrakt:	We report the development of an open-source Python application that provides quantitative and qualitative information from deconvoluted liquid-chromatography top-down mass spectrometry (LC-TDMS) data sets. This simple-to-use program allows users to search masses-of-interest across multiple LC-TDMS runs and provides visualization of their ion intensities and elution characteristics while quantifying their abundances relative to one another. Focusing on proteoform-rich histone proteins from the green microalga Chlamydomonas reinhardtii , we were able to quantify proteoform abundances across different growth conditions and replicates in minutes instead of hours typically needed for manual spreadsheet-based analysis. This resulted in extending previously published qualitive observations on Chlamydomonas histone proteoforms into quantitative ones, leading to an exciting new discovery on alpha-amino termini processing exclusive to histone H2A family members. Lastly, the script was intentionally developed with readability and customizability in mind so that fellow mass spectrometrists can modify the code to suit their lab-specific needs.
Databáze:	MEDLINE
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