Single-nucleus atlas of the Artemia female reproductive system suggests germline repression of the Z chromosome.

Autor: Elkrewi M; Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria., Vicoso B; Institute of Science and Technology Austria (ISTA), Klosterneuburg, Austria.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2024 Aug 30; Vol. 20 (8), pp. e1011376. Date of Electronic Publication: 2024 Aug 30 (Print Publication: 2024).
DOI: 10.1371/journal.pgen.1011376
Abstrakt: Our understanding of the molecular pathways that regulate oogenesis and define cellular identity in the Arthropod female reproductive system and the extent of their conservation is currently very limited. This is due to the focus on model systems, including Drosophila and Daphnia, which do not reflect the observed diversity of morphologies, reproductive modes, and sex chromosome systems. We use single-nucleus RNA and ATAC sequencing to produce a comprehensive single nucleus atlas of the adult Artemia franciscana female reproductive system. We map our data to the Fly Cell Atlas single-nucleus dataset of the Drosophila melanogaster ovary, shedding light on the conserved regulatory programs between the two distantly related Arthropod species. We identify the major cell types known to be present in the Artemia ovary, including germ cells, follicle cells, and ovarian muscle cells. Additionally, we use the germ cells to explore gene regulation and expression of the Z chromosome during meiosis, highlighting its unique regulatory dynamics and allowing us to explore the presence of meiotic sex chromosome silencing in this group.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Elkrewi, Vicoso. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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