A novel technique for atraumatic transurethral catheterisation of male rats.
| Autor: | Zhang SL; Plastic, Reconstructive and Aesthetic Surgery Service, Department of General Surgery, Woodlands Health, Singapore 737628, Singapore., Wong AW; Plastic, Reconstructive & Aesthetic Surgery Service, Sengkang General Hospital, Singapore 544886, Singapore. |
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| Jazyk: | angličtina |
| Zdroj: | Biology open [Biol Open] 2024 Sep 15; Vol. 13 (9). Date of Electronic Publication: 2024 Aug 30. |
| DOI: | 10.1242/bio.060476 |
| Abstrakt: | Transurethral catheterisation of male rats is technically difficult owing to anatomical peculiarities. In the male rat, the urethral striated sphincter consists of two lateral fascicles separated by an anterior and a posterior strip of connective tissue, which impedes the smooth insertion of a urinary catheter. For rat studies requiring continuous collection of urine, bladder irrigation, or measurement of bladder pressure, investigators either have to exclude the male population (be limited to the female population) or perform percutaneous (suprapubic) bladder puncture in male rats, which is more traumatic and invasive than transurethral catheterisation. This paper describes a novel, atraumatic method of transurethral catheterisation in the male rat, with the aid of a microscope and microsurgical instruments. Six Wistar rats were used for this experiment, all of which were catheterised successfully, with no evidence of bladder or urethral injury. The study shows that male rats can be safely catheterised via the urethra with the aid of a microscope and microsurgical instruments for both visual and tactile feedback. This is a relatively straightforward technique to learn and can allow for inclusion of male rats in future studies requiring urinary analysis or bladder irrigation, without the need for traumatic percutaneous (suprapubic) bladder puncture. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2024. Published by The Company of Biologists Ltd.) |
| Databáze: | MEDLINE |
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