Expression Levels and Clinical Values of miR-195, miR-424, miR-10b, miR-103a-3p, and miR-542-3p in Vasculo-Behçet's Disease.

Autor:	Kaymaz S; Department of Rheumatology., Aydın D; Department of Genetics., Uğur K; Department of Rheumatology., Çobankara V; Department of Rheumatology., Tan S; Department of Cancer Molecular Biology, Pamukkale University, Denizli, Turkey.
Jazyk:	angličtina
Zdroj:	Mediterranean journal of rheumatology [Mediterr J Rheumatol] 2023 Sep 07; Vol. 35 (2), pp. 255-262. Date of Electronic Publication: 2023 Sep 07 (Print Publication: 2024).
DOI:	10.31138/mjr.030623.elc
Abstrakt:	Objective: MicroRNAs (miRNAs) are involved in a range of pathological and biological processes. Vascular involvement is an important complication associated with morbidity and mortality in Behçet's disease (BD). In this study, we aimed to evaluate the expression levels of miR-195, miR-424, miR-10b, miR-103a-3p, and miR-542-3p in Turkish patients with BD, and their possible association with vascular involvement and clinical activity. Methods: This cross-sectional study included 61 BD patients and 25 age- and sex-matched healthy individuals. The patients were categorised into two groups based on the presence or absence of vascular involvement. Demographic data, disease duration, disease activity, and medical treatments were recorded. Disease activity was evaluated using the Behçet's Disease Current Activity Form (BDCAF) and the Behçet's Syndrome Activity Scale (BSAS). The expression levels of miRNAs were measured using real-time quantitative polymerase chain reaction (RT-qPCR). Results: The comparison of the clinical features of BD patients with and without vascular involvement revealed no significant difference. However, the expression levels of miR-195, miR-424, miR-10b, miR-103a-3p, and miR-542-3p were significantly higher in BD patients than in healthy controls (p<0.001, p<0.001, p=0.010, p<0.01, p=0.039, respectively). Moreover, the expression level of miR-195 was significantly higher in vasculo-Behçet patients than in the other groups (p=0.0318). However, no significant association was found between the expression levels of miR-195 and clinical activity. Conclusion: Our study results indicated elevated serum levels of miR-195 in BD patients, which may be associated with vascular involvement. Therefore, miR-195 could potentially serve as a biomarker for the diagnosis and monitoring of vasculo-Behçet's disease. Competing Interests: The authors declare no conflict of interest. (© 2024 The Mediterranean Journal of Rheumatology (MJR).)
Databáze:	MEDLINE
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