YY1-controlled regulatory connectivity and transcription are influenced by the cell cycle.

Autor: Lam JC; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Aboreden NG; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Midla SC; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA., Wang S; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA., Huang A; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA., Keller CA; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA.; Genomics Research Incubator, Pennsylvania State University, University Park, PA, USA., Giardine B; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA., Henderson KA; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA., Hardison RC; Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA., Zhang H; Institute of Molecular Physiology, Shenzhen Bay Laboratory, Shenzhen, China., Blobel GA; Division of Hematology, The Children's Hospital of Philadelphia, Philadelphia, PA, USA. blobel@email.chop.edu.; Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. blobel@email.chop.edu.
Jazyk: angličtina
Zdroj: Nature genetics [Nat Genet] 2024 Sep; Vol. 56 (9), pp. 1938-1952. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1038/s41588-024-01871-y
Abstrakt: Few transcription factors have been examined for their direct roles in physically connecting enhancers and promoters. Here acute degradation of Yin Yang 1 (YY1) in erythroid cells revealed its requirement for the maintenance of numerous enhancer-promoter loops, but not compartments or domains. Despite its reported ability to interact with cohesin, the formation of YY1-dependent enhancer-promoter loops does not involve stalling of cohesin-mediated loop extrusion. Integrating mitosis-to-G1-phase dynamics, we observed partial retention of YY1 on mitotic chromatin, predominantly at gene promoters, followed by rapid rebinding during mitotic exit, coinciding with enhancer-promoter loop establishment. YY1 degradation during the mitosis-to-G1-phase interval revealed a set of enhancer-promoter loops that require YY1 for establishment during G1-phase entry but not for maintenance in interphase, suggesting that cell cycle stage influences YY1's architectural function. Thus, as revealed here for YY1, chromatin architectural functions of transcription factors can vary in their interplay with CTCF and cohesin as well as by cell cycle stage.
(© 2024. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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