A chemical approach to extend flower longevity of Japanese morning glory via inhibition of master senescence regulator EPHEMERAL1.

Autor: Shibuya K; Institute of Vegetable and Floriculture Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan. shibuken@affrc.go.jp., Nozawa A; Proteo-Science Center (PROS), Ehime University, Matsuyama, Japan. nozawa.akira.my@ehime-u.ac.jp., Takahashi C; Proteo-Science Center (PROS), Ehime University, Matsuyama, Japan., Sawasaki T; Proteo-Science Center (PROS), Ehime University, Matsuyama, Japan.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2024 Sep; Vol. 10 (9), pp. 1377-1388. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1038/s41477-024-01767-z
Abstrakt: Petal senescence in flowering plants is a type of programmed cell death with highly regulated onset and progression. A NAM/ATAF1,2/CUC2 transcription factor, EPHEMERAL1 (EPH1), has been identified as a key regulator of petal senescence in Japanese morning glory (Ipomoea nil). Here we used a novel chemical approach to delay petal senescence in Japanese morning glory by inhibiting the DNA-binding activity of EPH1. A cell-free high-throughput screening system and subsequent bioassays found two tetrafluorophthalimide-based compounds, Everlastin1 and Everlastin2, that inhibited the EPH1-DNA interaction and delayed petal senescence. The inhibitory mechanism was due to the suppression of EPH1 dimerization. RNA-sequencing analysis revealed that the chemical treatment strongly suppressed the expression of programmed cell death- and autophagy-related genes. These results suggest that a chemical approach targeting a transcription factor can regulate petal senescence.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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