Interaction of high lipogenic states with titanium on osteogenesis.

Autor: Pinto TS; Lab. of Bioassays and Cellular Dynamics, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP, São Paulo State University, Botucatu, SP, Brazil., van der Eerden BC; Department of Internal Medicine, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., Schreuders-Koedam M; Department of Internal Medicine, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., van de Peppel J; Department of Internal Medicine, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., Ayada I; Department of Gastroenterology and Hepatology, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., Pan Q; Department of Gastroenterology and Hepatology, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., Verstegen MM; Department of Surgery, Erasmus MC, Erasmus University Medical Center, Rotterdam, Netherlands., van der Laan LJ; Department of Surgery, Erasmus MC, Erasmus University Medical Center, Rotterdam, Netherlands., Fuhler GM; Department of Gastroenterology and Hepatology, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands., Zambuzzi WF; Lab. of Bioassays and Cellular Dynamics, Department of Chemical and Biological Sciences, Institute of Biosciences, UNESP, São Paulo State University, Botucatu, SP, Brazil. Electronic address: w.zambuzzi@unesp.br., Peppelenbosch MP; Department of Gastroenterology and Hepatology, Erasmus MC, Erasmus University Medical Center, Rotterdam, the Netherlands.
Jazyk: angličtina
Zdroj: Bone [Bone] 2024 Nov; Vol. 188, pp. 117242. Date of Electronic Publication: 2024 Aug 28.
DOI: 10.1016/j.bone.2024.117242
Abstrakt: As obesity rates continue to rise, the prevalence of metabolic dysfunction and alcohol-associated steatotic liver disease (MetALD), a new term for Nonalcoholic Fatty Liver Disease (NAFLD), also increases. In an aging population, it is crucial to understand the interplay between metabolic disorders, such as MetALD, and bone health. This understanding becomes particularly significant in the context of implant osseointegration. This study introduces an in vitro model simulating high lipogenesis through the use of human Mesenchymal Stroma Cells-derived adipocytes, 3D intrahepatic cholangiocyte organoids (ICO), and Huh7 hepatocytes, to evaluate the endocrine influence on osteoblasts interacting with titanium. We observed a significant increase in intracellular fat accumulation in all three cell types, along with a corresponding elevation in metabolic gene expression compared to the control groups. Notably, osteoblasts undergoing mineralization in this high-lipogenesis environment also displayed lipid vesicle accumulation. The study further revealed that titanium surfaces modulate osteogenic gene expression and impact cell cycle progression, cell survival, and extracellular matrix remodeling under lipogenic conditions. These findings provide new insights into the challenges of implant integration in patients with obesity and MetALD, offering a deeper understanding of the metabolic influences on bone regeneration and implant success.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: