Glucose Oxidase-Coated Calcium Peroxide Nanoparticles as an Innovative Catalyst for In Situ H 2 O 2 -Releasing Hydrogels.

Autor: Thi PL; Institute of Applied Materials Science, Vietnam Academy of Science and Technology, Ho Chi Minh, 7000000, Vietnam.; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Ho Chi Minh, 7000000, Vietnam., Tu QA; Department of Molecular Science and Technology, Ajou University, 5 Woncheon, Yeongtong, Suwon, 16499, Republic of Korea., Oh DH; Department of Molecular Science and Technology, Ajou University, 5 Woncheon, Yeongtong, Suwon, 16499, Republic of Korea., Park KD; Department of Molecular Science and Technology, Ajou University, 5 Woncheon, Yeongtong, Suwon, 16499, Republic of Korea.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2024 Aug 29, pp. e2400268. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1002/mabi.202400268
Abstrakt: In situ forming and hydrogen peroxide (H 2 O 2 )-releasing hydrogels have been considered as attractive matrices for various biomedical applications. Particularly, horseradish peroxidase (HRP)-catalyzed crosslinking reaction serves efficient method to create in situ forming hydrogels due to its advantageous features, such as mild reaction conditions, rapid gelation rate, tunable mechanical strength, and excellent biocompatibility. Herein, a novel HRP-crosslinked hydrogel system is reported that can produce H 2 O 2 in situ for long-term applications, using glucose oxidase-coated calcium peroxide nanoparticles (CaO 2 @GOx NPs). In this system, CaO 2 gradually produced H 2 O 2 to support the HRP-mediated hydrogelation, while GOx further catalyzed the oxidation of glucose for in situ H 2 O 2 generation. As the hydrogel is formed rapidly is expected and the H 2 O 2 release behavior is prolonged up to 10 days. Interestingly, hydrogels formed by HRP/CaO 2 @GOx-mediated crosslinking reaction provided a favorable 3D microenvironment to support the viability and proliferation of fibroblasts, compared to that of hydrogels formed by either HRP/H 2 O 2 or HRP/CaO 2 /GOx-mediated crosslinking reaction. Furthermore, HRP/CaO 2 @GOx-crosslinked hydrogel enhanced the angiogenic activities of endothelial cells, which is demonstrated by the in vitro tube formation test and in ovo chicken chorioallantoic membrane model. Therefore, HRP/CaO 2 @GOx-catalyzed hydrogels is suggested as potential in situ H 2 O 2 -releasing materials for a wide range of biomedical applications.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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