Physiological Responses to Acute Heat Stress in Rohu, Labeo rohita: Insights from Liver Proteomics.

Autor: Badhe MR; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Das P; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Sahoo S; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Paul A; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Sahoo PK; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Reddy RRK; Institute of Life Sciences, Bhubaneswar, 751023, India., Suryawanshi AR; Institute of Life Sciences, Bhubaneswar, 751023, India., Nandanpawar PC; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Das Mahapatra K; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India., Nagpure NS; ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India., Goswami M; ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India., Mohanty J; ICAR-Central Institute of Freshwater Aquaculture, Bhubaneswar, 751002, India. jmohantycifa@gmail.com.
Jazyk: angličtina
Zdroj: Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2024 Dec; Vol. 26 (6), pp. 1129-1142. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1007/s10126-024-10360-6
Abstrakt: Heat stress is a major problem in aquaculture species, causing changes in physiology such as decreased feed intake, growth rate, reproduction, and internal cellular damage, thereby affecting fish's health. The effects of an acute heat stress simulating a daily rise and fall in temperature on summer days were evaluated in the liver proteome of rohu (Labeo rohita) fingerlings in the present study. The fish maintained at 30 °C were gradually exposed to a higher temperature of 36 °C at an increment rate of 1 °C per 1.5 h, and after 3 h at that temperature, it was gradually reduced to 30 °C. The liver tissue samples were collected at 5 am, 5 pm, and 5 am the next day from the exposed and control fish. Protein samples were prepared from the liver tissues, and the extracted proteins were compared using 2-dimensional (2D) gel electrophoresis (2DGE) and mass spectrometry (MS) using a MALDI-TOF/TOF mass spectrometer. A total of 44 differentially expressed protein spots were visualized in 2D gel analysis from heat stress exposed fish at three time points, out of which 21 proteins including one hypothetical protein could be identified by MS. The abundance of five selected differentially expressed proteins (DEPs) was validated using qPCR. The majority of DEPs were found to be involved primarily in lipid, protein and energy metabolism, immune system regulation, cytoskeletal stability, and ROS management. The findings of this study would help in the development of strategies to mitigate heat stress in L. rohita.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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