Spatial and temporal tracking of multi-layered cells sheet using reporter gene imaging with human sodium iodide symporter: a preclinical study using a rat model of myocardial infarction.

Autor: Otani K; Department of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan., Zeniya T; Graduate School of Science and Technology, Hirosaki University, Aomori, Japan., Kawashima H; Radioisotope Research Center, Kyoto Pharmaceutical University, Kyoto, Japan., Moriguchi T; Tandem Accelerator Complex (UTTAC), University of Tsukuba, Ibaraki, Japan., Nakano A; Department of Research Promotion and Management, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan., Han C; Turku PET Centre, Turku University Hospital, Turku, Finland., Murata S; Department of Preventive Medicine and Epidemiology, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan., Nishimura K; Department of Preventive Medicine and Epidemiology, National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan., Koshino K; Department of Systems and Informatics, Hokkaido Information University, Hokkaido, Japan., Yamahara K; Laboratory of Molecular and Cellular Therapy, Institute for Advanced Medical Sciences, Hyogo Medical University, Hyogo, Japan., Inubushi M; Division of Nuclear Medicine, Department of Radiology, Kawasaki Medical School, Okayama, Japan., Iida H; Turku PET Centre, Turku University Hospital, Turku, Finland. hidehiro.iida@utu.fi.; Turku PET Centre, University of Turku and Turku University Hospital, Building 14, Kiinamyllynkatu 4-8, Turku, 20520, Finland. hidehiro.iida@utu.fi.
Jazyk: angličtina
Zdroj: European journal of nuclear medicine and molecular imaging [Eur J Nucl Med Mol Imaging] 2024 Dec; Vol. 52 (1), pp. 74-87. Date of Electronic Publication: 2024 Aug 29.
DOI: 10.1007/s00259-024-06889-2
Abstrakt: Purpose: This study aimed to evaluate a novel technique for cell tracking by visualising the activity of the human sodium/iodide symporter (hNIS) after transplantation of hNIS-expressing multilayered cell sheets in a rat model of chronic myocardial infarction.
Methods: Triple-layered cell sheets were generated from mouse embryonic fibroblasts (MEFs) derived from mice overexpressing hNIS (hNIS-Tg). Myocardial infarction was induced by permanent ligation of the left anterior descending coronary artery in F344 athymic rats, and a triple-layered MEFs sheets were transplanted to the infarcted area two weeks after surgery. To validate the temporal tracking and kinetic analysis of the transplanted MEFs sheets, sequential cardiac single-photon emission computed tomography (SPECT) examinations with a 99m TcO 4 - injection were performed. The cell sheets generated using MEFs of wild-type mice (WT) served as controls.
Results: A significantly higher amount of 99m TcO 4 - was taken into the hNIS-Tg MEFs than into WT MEFs (146.1 ± 30.9-fold). The obvious accumulation of 99m TcO 4 - was observed in agreement with the region where hNIS-Tg MEFs were transplanted, and these radioactivities peaked 40-60 min after 99m TcO 4 - administration. The volume of distribution of the hNIS-Tg MEF sheets declined gradually after transplantation, implying cellular malfunction and a loss in the number of transplanted cells.
Conclusion: The reporter gene imaging with hNIS enables the serial tracking and quantitative kinetic analysis of cell sheets transplanted to infarcted hearts.
Competing Interests: Declarations. Competing interests: The authors have no relevant financial or nonfinancial interests to disclose. Ethics approval: The animal study was approved by the National Cerebral and Cardiovascular Center Ethics Committee for Animal Experiments and was conducted in accordance with the guidelines of the Physiological Society of Japan.
(© 2024. The Author(s).)
Databáze: MEDLINE
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