Characterization of a decellularized pericardium extracellular matrix hydrogel for regenerative medicine: insights on animal-to-animal variability.

Autor:	Di Francesco D; Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec, QC, Canada.; Department of Health Sciences, University of Piemonte Orientale 'A. Avogadro', Novara, Italy., Marcello E; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Torino, Italy.; Polito BioMed Lab, Politecnico di Torino, Torino, Italy., Casarella S; Department of Health Sciences, University of Piemonte Orientale 'A. Avogadro', Novara, Italy., Copes F; Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec, QC, Canada., Chevallier P; Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec, QC, Canada., Carmagnola I; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Torino, Italy.; Polito BioMed Lab, Politecnico di Torino, Torino, Italy., Mantovani D; Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec, QC, Canada., Boccafoschi F; Department of Health Sciences, University of Piemonte Orientale 'A. Avogadro', Novara, Italy.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2024 Aug 14; Vol. 12, pp. 1452965. Date of Electronic Publication: 2024 Aug 14 (Print Publication: 2024).
DOI:	10.3389/fbioe.2024.1452965
Abstrakt:	In the past years, the use of hydrogels derived from decellularized extracellular matrix (dECM) for regenerative medicine purposes has significantly increased. The intrinsic bioactive and immunomodulatory properties indicate these materials as promising candidates for therapeutical applications. However, to date, limitations such as animal-to-animal variability still hinder the clinical translation. Moreover, the choice of tissue source, decellularization and solubilization protocols leads to differences in dECM-derived hydrogels. In this context, detailed characterization of chemical, physical and biological properties of the hydrogels should be performed, with attention to how these properties can be affected by animal-to-animal variability. Herein, we report a detailed characterization of a hydrogel derived from the decellularized extracellular matrix of bovine pericardium (dBP). Protein content, rheological properties, injectability, surface microstructure, in vitro stability and cytocompatibility were evaluated, with particular attention to animal-to-animal variability. The gelation process showed to be thermoresponsive and the obtained dBP hydrogels are injectable, porous, stable up to 2 weeks in aqueous media, rapidly degrading in enzymatic environment and cytocompatible, able to maintain cell viability in human mesenchymal stromal cells. Results from proteomic analysis proved that dBP hydrogels are highly rich in composition, preserving bioactive proteoglycans and glycoproteins in addition to structural proteins such as collagen. With respect to the chemical composition, animal-to-animal variability was shown, but the biological properties were not affected, which remained consistent in different batches. Taken together these results show that dBP hydrogels are excellent candidates for regenerative medicine applications. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. The authors declare that this study received funding from Tissuegraft Srl. The funder had the following involvement in the study: supply of primary material samples (decellularized extracellular matrix hydrogels) for the study and funded third party services. (Copyright © 2024 Di Francesco, Marcello, Casarella, Copes, Chevallier, Carmagnola, Mantovani and Boccafoschi.)
Databáze:	MEDLINE
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