Exploring the antimicrobial potential of crude peptide extracts from Allium sativum and Allium oschaninii against antibiotic-resistant bacterial strains.

Autor:	Swangsri T; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Reamtong O; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Saralamba S; Mathematical and Economic Modelling (MAEMOD), Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Rakthong P; Faculty of Science and Technology, Rajabhat Suratthani University, Surat Thani, Thailand., Thaenkham U; Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand., Saralamba N; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Jazyk:	angličtina
Zdroj:	Pharmaceutical biology [Pharm Biol] 2024 Dec; Vol. 62 (1), pp. 666-675. Date of Electronic Publication: 2024 Aug 28.
DOI:	10.1080/13880209.2024.2395517
Abstrakt:	Context: Plant peptides garner attention for their potential antimicrobial properties amid the rising concern over antibiotic-resistant bacteria. Objective: This study investigates the antibacterial potential of crude peptide extracts from 27 Thai plants collected locally. Materials and Methods: Peptide extracts from 34 plant parts, derived from 27 Thai plants, were tested for their antimicrobial efficacy against four highly resistant bacterial strains: Streptococcus aureus MRSA, Pseudomonas aeruginosa , Acinetobacter baumannii , and Escherichia coli . The stability of these peptide extracts was examined at different temperatures, and the synergistic effects of two selected plant peptide extracts were investigated. Additionally, the time-kill kinetics of the individual extracts and their combination were determined against the tested pathogens. Results: Peptides from Allium sativum L. and Allium oschaninii O. Fedtsch (Amaryllidaceae) were particularly potent, inhibiting bacterial growth with MICs ranging from 1.43 to 86.50 µg/mL. The consistent MICs and MBCs of these extracts across various extraction time points highlight their reliability. Stability tests reveal that these peptides maintain their antimicrobial activity at -20 °C for over a month, emphasizing their durability for future exploration and potential applications in addressing antibiotic resistance. Time-kill assays elucidate the time and concentration-dependent nature of these antimicrobial effects, underscoring their potent initial activity and sustained efficacy over time. Discussion and Conclusions: This study highlights the antimicrobial potential of Allium -derived peptides, endorsing them for combating antibiotic resistance and prompting further investigation into their mechanisms.
Databáze:	MEDLINE
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