Tuning viscoelasticity and stiffness in bioprinted hydrogels for enhanced 3D cell culture: A multi-scale mechanical analysis.

Autor: Pragnere S; Laboratory of Tribology and System Dynamics UMR-CNRS 5513, Ecole Centrale de, Lyon, France; Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600, MB, Eindhoven, the Netherlands., Courtial EJ; 3d.FAB, Univ Lyon, Université Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd Du 11, Villeurbanne cedex, France., Dubreuil F; Laboratory of Tribology and System Dynamics UMR-CNRS 5513, Ecole Centrale de, Lyon, France., Errazuriz-Cerda E; Centre D'Imagerie Quantitative Lyon-Est (CIQLE), Université Claude Bernard Lyon 1, Lyon, France., Marquette C; 3d.FAB, Univ Lyon, Université Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd Du 11, Villeurbanne cedex, France., Petiot E; 3d.FAB, Univ Lyon, Université Lyon 1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, 43, Bd Du 11, Villeurbanne cedex, France., Pailler-Mattei C; Laboratory of Tribology and System Dynamics UMR-CNRS 5513, Ecole Centrale de, Lyon, France; University of Lyon, Université Claude Bernard Lyon 1, ISPB-Faculté de Pharmacie de, Lyon, France. Electronic address: cyril.pailler-mattei@ec-lyon.fr.
Jazyk: angličtina
Zdroj: Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2024 Nov; Vol. 159, pp. 106696. Date of Electronic Publication: 2024 Aug 22.
DOI: 10.1016/j.jmbbm.2024.106696
Abstrakt: Bioprinted hydrogels are extensively studied to provide an artificial matrix for 3D cell culture. The success of bioprinting hydrogels relies on fine-tuning their rheology and composition to achieve shear-thinning behavior. However, a challenge arises from the limited viscoelastic and stiffness range accessible from a single hydrogel formulation. Nevertheless, hydrogel mechanical properties are recognized as essential cues influencing cell phenotype, migration, and differentiation. Thus, it is crucial to develop a system to easily modulate bioprinted hydrogels' mechanical behaviors. In this work, we modulated the viscoelastic properties and stiffness of bioprinted hydrogels composed of fibrinogen, alginate, and gelatin by tuning the crosslinking bath solution. Various concentrations of calcium ionically crosslinked alginate, while transglutaminase crosslinked gelatin. Subsequently, we characterized the mechanical behavior of our bioprinted hydrogels from the nanoscale to the macroscale. This approach enabled the production of diverse bioprinted constructs, either with similar elastic behavior but different elastic moduli or with similar elastic moduli but different viscoelastic behavior from the same hydrogel formulation. Culturing fibroblasts in the hydrogels for 33 days revealed a preference for cell growth and matrix secretion in the viscoelastic hydrogels. This work demonstrates the suitability of the method to decouple the effects of material mechanical from biochemical composition cues on 3D cultured cells.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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