| Autor: |
Martins JCL; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; CECAV-Veterinary and Animal Research Centre, Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.; AquaValor-Centro de Valorização e Transferência de Tecnologia da Água, 5400-342 Chaves, Portugal., Gonçalves A; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; CECAV-Veterinary and Animal Research Centre, Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal., Fernandes C; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CIMO-Centro de Investigação de Montanha, SusTEC-Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha, IPB-Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal., Cabecinha E; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; CIBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, 4485-661Vairão, Portugal., Monteiro S; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal., Guedes H; National Institute for Agricultural and Veterinary Research I.P. (INIAV), Lugar da Madalena, 4485-655 Vila do Conde, Portugal., Almeida G; National Institute for Agricultural and Veterinary Research I.P. (INIAV), Lugar da Madalena, 4485-655 Vila do Conde, Portugal.; Centre for Study in Animal Science (CECA-ICETA), Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Porto, 4099-002 Porto, Portugal., Garcia J; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; AquaValor-Centro de Valorização e Transferência de Tecnologia da Água, 5400-342 Chaves, Portugal., da Silva GJ; Faculty of Pharmacy, Center for Neurosciences and Cell Biology, University of Coimbra, 3000-548 Coimbra, Portugal., Varandas S; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; CIBIO-Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Universidade do Porto, Campus de Vairão, 4485-661Vairão, Portugal., Saavedra MJ; AB2Unit-Antimicrobials, Biocides & Biofilms Unit, Veterinary Sciences Department, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, Portugal.; CITAB-Centre for the Research and Technology of Agro-Environment and Biological Sciences, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production (Inov4Agro), University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.; CECAV-Veterinary and Animal Research Centre, Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. |
| Abstrakt: |
As bioindicators, freshwater bivalves are crucial for the assessment of the contamination impact on different levels of biological integration. Escherichia coli is used as a bioindicator of water fecal contamination, representing a critical global concern, especially with the rise of multidrug-resistant (MDR) strains. Phylogenetic diversity, pathotypic characterization, and antibiotic resistance profiles of E. coli isolated from freshwater bivalves ( Anodonta anatina ) were assessed. Samples were collected from the Tua River in Northern Portugal, from two different sites, Chelas and Barcel, representing different degrees of contamination. Antimicrobial susceptibility testing was performed by the disk diffusion method, and characterizations of the phylogenetic groups and pathotypes were assessed by PCR-multiplex and real-time PCR-multiplex, respectively. Results showed that 60% of isolates were characterized as MDR, including resistance to carbapenems, considered the last resort against multidrug-resistant bacteria. Within this study, it was also possible to verify the antimicrobial resistance (AMR) profile differences between the two sampling sites, with bivalve isolates from the Chelas site showing a higher percentage of antibiotic resistance. Among the E. coli isolates, the highest prevalence (55%) was observed in group B1, followed by group D or E (15%), group A (10%), and group E or Clade I (10%). None of the isolates were classified as diarrheagenic E. coli (DEC). This work highlights the potential transmission of antimicrobial-resistant bacteria through bivalves in the food chain. The 'One Health' approach is crucial for combating antimicrobial resistance, namely in edible freshwater species, emphasizing active surveillance to protect human, animal, and environmental health against the spread of antibiotic-resistant bacteria in aquatic environments. |
