| Autor: |
Martínez-Sánchez V; Group of Chemistry and Biochemistry of Pigments, Instituto de la Grasa (CSIC), Building 46, 41013 Sevilla, Spain., Calvo MV; Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CSIC-UAM), 28049 Madrid, Spain., Fontecha J; Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CSIC-UAM), 28049 Madrid, Spain., Pérez-Gálvez A; Group of Chemistry and Biochemistry of Pigments, Instituto de la Grasa (CSIC), Building 46, 41013 Sevilla, Spain. |
| Abstrakt: |
This study aimed to evaluate the digestive efficiency of food matrices supplemented with milk fat globule membrane isolated from buttermilk (BM-MFGM), using the INFOGEST in vitro digestion protocol hyphenated with the assessment of the digested material on the lipid profile of the Caco-2 cell culture model. First, we examined lipid profiles in food matrices supplemented with BM-MFGM and their subsequent digestion. The results showed distinct lipid profiles in different food matrices and micellar fractions. The presence of BM-MFGM lipids changed the cellular lipid profiles in Caco-2 cell cultures, with diverging contents in cholesteryl esters, triacylglycerides, and neutral lipids depending on the micellar food matrix factor. Hierarchical clustering analysis revealed patterns in cellular lipid responses to micellar stimuli, while volcano plots highlighted significant changes in cellular lipid profiles post-treatment. Thus, this study underscores the importance of in vitro digestion protocols in guiding food matrix selection for bioactive ingredient supplementation, elucidating intestinal epithelium responses to digested food stimuli. |
