| Autor: |
Rodriguez I; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Nam YH; Invivotec Co., Ltd., Seongnam 13449, Republic of Korea., Shin SW; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Seo GJ; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Kim NW; Invivotec Co., Ltd., Seongnam 13449, Republic of Korea., Nuankaew W; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Kim DH; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Park YH; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Lee HY; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea., Peng XH; Department of Garden, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun 666303, China., Hong BN; Invivotec Co., Ltd., Seongnam 13449, Republic of Korea., Kang TH; Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Global Campus, Yongin 17104, Republic of Korea. |
| Abstrakt: |
Sensorineural hearing loss (SNHL), characterized by damage to the inner ear or auditory nerve, is a prevalent auditory disorder. This study explores the potential of Castanopsis echinocarpa (CAE) as a therapeutic agent for SNHL. In vivo experiments were conducted using zebrafish and mouse models. Zebrafish with neomycin-induced ototoxicity were treated with CAE, resulting in otic hair cell protection with an EC 50 of 0.49 µg/mL and a therapeutic index of 1020. CAE treatment improved auditory function and protected cochlear sensory cells in a mouse model after noise-induced hearing loss (NIHL). RNA sequencing of NIHL mouse cochleae revealed that CAE up-regulates genes involved in neurotransmitter synthesis, secretion, transport, and neuronal survival. Real-time qPCR validation showed that NIHL decreased the mRNA expression of genes related to neuronal function, such as Gabra1 , Gad1 , Slc32a1 , CaMK2b , CaMKIV , and Slc17a7 , while the CAE treatment significantly elevated these levels. In conclusion, our findings provide strong evidence that CAE protects against hearing loss by promoting sensory cell protection and enhancing the expression of genes critical for neuronal function and survival. |
