| Autor: |
Karlsson A; Nanoxis Consulting AB, 40016 Gothenburg, Sweden., Alarcón LA; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 40530 Gothenburg, Sweden., Piñeiro-Iglesias B; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 40530 Gothenburg, Sweden.; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, 41345 Gothenburg, Sweden., Jacobsson G; Department of Infectious Diseases, Skaraborg Hospital, 54185 Skövde, Sweden.; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, 40530 Gothenburg, Sweden., Skovbjerg S; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 40530 Gothenburg, Sweden.; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, 41345 Gothenburg, Sweden.; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, 40530 Gothenburg, Sweden., Moore ERB; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 40530 Gothenburg, Sweden.; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, 41345 Gothenburg, Sweden.; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, 40530 Gothenburg, Sweden.; Culture Collection of the University of Gothenburg (CCUG), Sahlgrenska Academy, 41390 Gothenburg, Sweden., Kopparapu PK; Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 41390 Gothenburg, Sweden.; Department of Rheumatology, Sahlgrenska University Hospital, 41345 Gothenburg, Sweden., Jin T; Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 41390 Gothenburg, Sweden.; Department of Rheumatology, Sahlgrenska University Hospital, 41345 Gothenburg, Sweden., Karlsson R; Nanoxis Consulting AB, 40016 Gothenburg, Sweden.; Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, 40530 Gothenburg, Sweden.; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, 41345 Gothenburg, Sweden.; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, 40530 Gothenburg, Sweden. |
| Abstrakt: |
Staphylococcus aureus is a pathogen known to cause a wide range of infections. To find new targets for identification and to understand host-pathogen interactions, many studies have focused on surface proteins. We performed bacterial-cell surface-shaving, followed by tandem mass tag for quantitative mass spectrometry proteomics, to examine the surfaceome of S. aureus . Two steps were performed, the first step including surface protein-deficient mutants of S. aureus Newman strain lacking important virulence genes ( clfA and spa , important for adhesion and immune evasion and srtAsrtB , linking surface-associated virulence factors to the surface) and the second step including isolates of different clinical origin. All strains were compared to the Newman strain. In Step 1, altogether, 7880 peptides were identified, corresponding to 1290 proteins. In Step 2, 4949 peptides were identified, corresponding to 919 proteins and for each strain, approximately 20 proteins showed differential expression compared to the Newman strain. The identified surface proteins were related to host-cell-adherence and immune-system-evasion, biofilm formation, and survival under harsh conditions. The results indicate that surface-shaving of intact S. aureus bacterial strains in combination with quantitative proteomics is a useful tool to distinguish differences in protein abundance of the surfaceome, including the expression of virulence factors. |
