| Autor: |
Bastida-Ramírez LJ; Facultad de Química, Universidad Autónoma del Estado de México, Toluca C.P. 50120, Mexico., Buendía-González L; Facultad de Ciencias, Universidad Autónoma del Estado de México, Toluca C.P. 50200, Mexico., Mejía-Argueta EL; Facultad de Química, Universidad Autónoma del Estado de México, Toluca C.P. 50120, Mexico., Sandoval-Cabrera A; Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca C.P. 50180, Mexico.; Laboratorio de Alta Especialidad Hemato-Oncología, Hospital para el Niño, Instituto Materno Infantil del Estado de México, Toluca C.P. 50170, Mexico., García-Fabila MM; Facultad de Química, Universidad Autónoma del Estado de México, Toluca C.P. 50120, Mexico., Pavón-Romero SH; Facultad de Química, Universidad Autónoma del Estado de México, Toluca C.P. 50120, Mexico., Padua-Ahumada M; Centro Médico, ISSEMyM, Toluca C.P. 52170, Mexico., Santillán-Benítez JG; Facultad de Química, Universidad Autónoma del Estado de México, Toluca C.P. 50120, Mexico. |
| Abstrakt: |
(1) Background: Could compounds such as monoterpenes and sesquiterpenes present in essential plant oils inhibit bacterial growth as an alternative to help mitigate bacterial resistance? The purpose of this study is evaluating the in vitro antibacterial effect of Lippia organoides EO (LEO) and Thymus vulgaris EO (TEO), individually and in combination with ampicillin, against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains; (2) Methods: Experimental in vitro design with post-test. The EOs were obtained by hydrodistillation and were analyzed by GC. ESBL-producing E. coli strains used were selected from urine cultures and the bla CTX-M and bla TEM resistance genes were identified by end point PCR. The disk diffusion method was used for the susceptibility tests. The MICs and MBCs were determined by microdilution test. Finally, the interaction effect was observed by checkerboard assay; (3) Results: A 39.9% decrease in the growth of the strain thymol in TEO and 70.4% in carvacrol in LEO was shown, observing inhibition halos of 32 mm for both EOs. MICs of 632 and 892 μg/mL for LEO and 738 and 940 μg/mL for TEO were determined. Finally, it was observed that, at low doses, there is a synergistic effect between TEO + LEO and EOs + ampicillin; (4) Conclusions: The findings demonstrate that TEO and LEO have an inhibitory effect on ESBL-producing E. coli , suggesting that they are candidates for further studies in the formulation of antibiotics to reduce bacterial resistance to traditional antibiotics. |
