| Autor: |
Trees E; Association of Public Health Laboratories, Bethesda, MD 20814, USA., Carleton HA; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Folster JP; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Gieraltowski L; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Hise K; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Leeper M; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Nguyen TA; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Poates A; Association of Public Health Laboratories, Bethesda, MD 20814, USA., Sabol A; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Tagg KA; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Tolar B; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Vasser M; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Webb HE; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Wise M; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA., Lindsey RL; Centers for Disease Control and Prevention, Atlanta, GA 30333, USA. |
| Abstrakt: |
Whole genome sequencing is replacing traditional laboratory surveillance methods as the primary tool to track and characterize clusters and outbreaks of the foodborne and zoonotic pathogen Salmonella enterica ( S. enterica ). In this study, 438 S. enterica isolates representing 35 serovars and 13 broad vehicle categories from one hundred epidemiologically confirmed outbreaks were evaluated for genetic variation to develop epidemiologically relevant interpretation guidelines for Salmonella disease cluster detection. The Illumina sequences were analyzed by core genome multi-locus sequence typing (cgMLST) and screened for antimicrobial resistance (AR) determinants and plasmids. Ninety-three of the one hundred outbreaks exhibited a close allele range (less than 10 allele differences with a subset closer than 5). The remaining seven outbreaks showed increased variation, of which three were considered polyclonal. A total of 16 and 28 outbreaks, respectively, showed variations in the AR and plasmid profiles. The serovars Newport and I 4,[5],12:i:-, as well as the zoonotic and poultry product vehicles, were overrepresented among the outbreaks, showing increased variation. A close allele range in cgMLST profiles can be considered a reliable proxy for epidemiological relatedness for the vast majority of S. enterica outbreak investigations. Variations associated with mobile elements happen relatively frequently during outbreaks and could be reflective of changing selective pressures. |
