| Autor: |
Alves-Silva JM; Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.; Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal., Zuzarte M; Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.; Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal., Salgueiro L; Faculty of Pharmacy, University of Coimbra, Azinhaga de S. Comba, 3000-548 Coimbra, Portugal.; Department of Chemical Engineering, Chemical Engineering and Renewable Resources for Sustainability (CERES), University of Coimbra, 3030-790 Coimbra, Portugal., Cocco E; Laboratory of Economic and Pharmaceutical Botany, Department of Life and Environmental Sciences, University of Cagliari, V.le S. Ignazio da Laconi 13, 09123 Cagliari, Italy., Ghiani V; Autonomous Region of Sardinia, 09124 Cagliari, Italy., Falconieri D; Laboratory of Economic and Pharmaceutical Botany, Department of Life and Environmental Sciences, University of Cagliari, V.le S. Ignazio da Laconi 13, 09123 Cagliari, Italy., Maccioni D; Laboratory of Economic and Pharmaceutical Botany, Department of Life and Environmental Sciences, University of Cagliari, V.le S. Ignazio da Laconi 13, 09123 Cagliari, Italy., Maxia A; Laboratory of Economic and Pharmaceutical Botany, Department of Life and Environmental Sciences, University of Cagliari, V.le S. Ignazio da Laconi 13, 09123 Cagliari, Italy. |
| Abstrakt: |
Artichokes ( Cynara scolymus L.) are valuable foods, thanks to their health benefits, but they generate significant waste during their production, harvesting, and processing, which poses sustainability issues. This study applied an agroprospecting approach to convert Tema artichoke biowaste (TB) into valuable resources, starting from a global perspective of the production chain to the targeted applications based on chemical and biological analysis. The major TB was identified in the outer bracts of the immature flower heads, which were collected throughout the harvesting season, extracted, and analyzed. The most abundant compounds were phenolic acids including chlorogenic acid and caffeoylquinic derivatives. Among flavonoids, cynaroside was the most abundant compound. Multivariate analysis distinguished batches by collection period, explaining 77.7% of the variance, with most compounds increasing in concentration later in the harvest season. Subsequently, TB extracts were analyzed for their potential in wound healing and anti-aging properties. Fibroblasts were used to assess the effect of selected extracts on cell migration through a scratch wound assay and on cellular senescence induced by etoposide. The results show a significant decrease in senescence-associated β-galactosidase activity, γH2AX nuclear accumulation, and both p53 and p21 protein levels. Overall, this study ascribes relevant anti-skin aging effects to TB, thus increasing its industrial value in cosmeceutical and nutraceutical applications. |
