Anti-Cataract Effect of the Traditional Aqueous Extract of Yerba Mate ( Ilex paraguariensis A. St.-Hil.): An In Ovo Perspective.

Autor: Oncu S; Department of Medical Pharmacology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Becit-Kizilkaya M; Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Bilir A; Department of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Saritas A; Department of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Arikan-Soylemez ES; Department of Medical Biology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Koca HB; Department of Biochemistry, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Firat F; Department of Histology and Embryology, Medicine Faculty, Afyonkarahisar Health Sciences University, Afyonkarahisar 03030, Turkey., Ugur-Kaplan AB; Department of Pharmaceutical Technology, Faculty of Pharmacy, Ataturk University, Erzurum 25240, Turkey., Yilmaz MA; Department of Analytical Chemistry, Faculty of Pharmacy, Dicle University, Diyarbakir 21280, Turkey.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2024 Aug 10; Vol. 14 (8). Date of Electronic Publication: 2024 Aug 10.
DOI: 10.3390/life14080994
Abstrakt: Introduction: The therapeutic effect of different doses of the traditional aqueous extract of dried leaves of yerba mate ( Ilex paraguariensis A. St.-Hil.) was investigated in an experimental cataract model in chicken embryos.
Methods and Results: LC-MS/MS analysis allowed the identification and quantification of 53 metabolites. In the hydrocortisone-induced cataract model, lenses were examined morphologically after treatment and parameters related to oxidative stress (total antioxidant/oxidant status (TAS/TOS), glutathione (GSH), and malondialdehyde (MDA)) were evaluated. Antiproliferative cell nuclear antigen (PCNA) and caspase-3 H-scores were determined and crystallin alpha A ( CRYAA ) gene expression in the lenses was measured by RT-PCR. The degree of cataract decreased in all treatment groups. While there was no significant difference in TAS levels compared to the negative control, TOS, GSH, and MDA levels were dose-dependently regulated. Treatment groups other than the high-dose group regulated the decrease in PCNA and the increase in caspase-3. CRYAA gene expression increased significantly only at the lowest dose.
Conclusion: YM, which is becoming increasingly popular as a traditional tea, showed a therapeutic effect on hydrocortisone-induced cataracts in chicken embryos at relatively low doses.
Databáze: MEDLINE
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