| Autor: |
Labied S; Center for Reproductive Medicine, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium., Wenders F; Center for Reproductive Medicine, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium., Gaspard O; Center for Reproductive Medicine, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium., Ravet S; Center for Reproductive Medicine, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium., Desmecht A; Faculty of Medicine, University of Liège, Place du Vingt Août 7, 4000 Liege, Belgium., Nisolle M; Obstetrics and Gynecology Department, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium., Henry L; Center for Reproductive Medicine, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium.; Obstetrics and Gynecology Department, University of Liège, Boulevard du 12ème de Ligne 1, 4000 Liege, Belgium. |
| Abstrakt: |
Background and Objectives: Fertilized zygotes normally display two pronuclei (PN), but abnormal fertilization patterns (0, 1 or >2PN) are observed daily in IVF labs. Multiple PN zygotes (>2) are generally discarded due to an increased risk of aneuploidy. However, the decision to transfer or not transfer 1PN-derived embryos remains controversial. The aims of our study were to analyze the neonatal outcomes of fresh or frozen-thawed embryos derived from 1PN zygotes, and to evaluate the influence of the fertilization method. Materials and Methods : Data were retrospectively collected from cycles performed between January 2018 and December 2022. Fresh cycles were analyzed for the comparative fate of 1PN zygotes (n = 1234) following conventional in vitro fertilization (cIVF; n = 648) or intracytoplasmic sperm injection (ICSI; n = 586), as well as the results of the 64 transfers of 1PN-derived embryos (pregnancy rate (PR) and neonatal outcomes). This pregnancy follow-up was also applied to 167 transfers of frozen-thawed 1PN-derived embryos. Results: In fresh cycles, 46% of the 1PN zygotes in the cIVF group developed into embryos of sufficient quality to be transferred or frozen (day 3 or 5/6). This rate was lower in the fresh ICSI cycles (33%). Blastulation rate was also significantly higher in the cIVF group (44%) in comparison to the ICSI group (20%). The fresh single embryo transfers (32 per group) allowed seven pregnancies in the cIVF group (PR = 21.9%) as compared to four pregnancies in the ICSI group (PR = 12.5%). In the cIVF group, five deliveries of healthy newborns were achieved, but only one in the ICSI group. In frozen/thawed cycles, 36 pregnancies were obtained out of the 167 transfers. A non-significant difference was observed between embryos derived from cIVF cycles (PR = 26%) and ICSI cycles (PR = 16%) with 18 and 8 healthy babies born, respectively. Conclusions: We observed better outcomes for 1PN zygotes in cIVF cycles in comparison to ICSI cycles. Our center policy to transfer good-quality 1PN-derived embryos allowed the birth of 32 healthy babies. |
