| Autor: |
Cerqueira Melo RC; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Martins AA; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Melo ALF; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Vicente JCP; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Sturaro MC; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Arantes JP; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Rossato L; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., de Souza GHA; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil., Simionatto S; Health Sciences Research Laboratory, Federal University of Grande Dourados (UFGD), Dourados 79804970, Mato Grosso do Sul, Brazil. |
| Abstrakt: |
Antimicrobial resistance (AMR) has emerged as a significant threat to public health, particularly in infections caused by critically important Gram-negative bacteria. The development of novel antibiotics has its limitations, and therefore it is crucial to explore alternative strategies to effectively combat infections with resistant pathogens. In this context, the present study investigated the antibacterial potency of 560 compounds against the multidrug-resistant (MDR) strains of Klebsiella pneumoniae and Serratia marcescens . The evaluated compounds were selected from the Pandemic Response Box (PRB) and COVID Box (CB) and subjected to assays to determine the inhibitory concentration (IC), minimum bactericidal concentration (MBC), and biofilm formation. Further, the effects of these compounds on membrane integrity were assessed through protein quantification. Several of the evaluated compounds, including fusidic acid, MMV1580853, and MMV1634399, exhibited a significant reduction in biofilm formation and growth in K. pneumoniae . Trimethoprim exhibited potential against S. marcescens . The IC values of the compounds indicated significant microbial growth inhibition at various concentrations. These findings underscore the potency of the existing antibiotics and novel compounds in combating the MDR strains of bacteria. The importance of reconsidering the known antibiotics and utilizing drug repositioning strategies to address the increasing risk of AMR is highlighted. |
