Single-cell RNA-seq Reveals the Inhibitory Effect of Methamphetamine on Liver Immunity with the Involvement of Dopamine Receptor D1.

Autor:	Zhou JT; Key Laboratory of National Health Commission for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.; National Biosafety Evidence Foundation, Bio-evidence Sciences Academy, Xi'an Jiaotong University, Xi'an 710115, China., Xu Y; Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China., Liu XH; Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China., Cheng C; Key Laboratory of National Health Commission for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.; National Biosafety Evidence Foundation, Bio-evidence Sciences Academy, Xi'an Jiaotong University, Xi'an 710115, China., Fan JN; Key Laboratory of National Health Commission for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.; National Biosafety Evidence Foundation, Bio-evidence Sciences Academy, Xi'an Jiaotong University, Xi'an 710115, China., Li X; National & Local Joint Engineering Research Center of Biodiagnosis and Biotherapy, Precision Medical Institute, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China., Yu J; National Biosafety Evidence Foundation, Bio-evidence Sciences Academy, Xi'an Jiaotong University, Xi'an 710115, China.; OneHealth Technology Company, Xi'an 710000, China., Li S; Key Laboratory of National Health Commission for Forensic Sciences, Xi'an Jiaotong University, Xi'an 710061, China.; National Biosafety Evidence Foundation, Bio-evidence Sciences Academy, Xi'an Jiaotong University, Xi'an 710115, China.
Jazyk:	angličtina
Zdroj:	Genomics, proteomics & bioinformatics [Genomics Proteomics Bioinformatics] 2024 Oct 15; Vol. 22 (4).
DOI:	10.1093/gpbjnl/qzae060
Abstrakt:	Methamphetamine (METH) is a highly addictive psychostimulant that causes physical and psychological damage and immune system disorder, especially in the liver which contains a significant number of immune cells. Dopamine, a key neurotransmitter in METH addiction and immune regulation, plays a crucial role in this process. Here, we developed a chronic METH administration model and conducted single-cell RNA sequencing (scRNA-seq) to investigate the effect of METH on liver immune cells and the involvement of dopamine receptor D1 (DRD1). Our findings reveal that chronic exposure to METH induces immune cell identity shifts from IFITM3+ macrophage (Mac) and CCL5+ Mac to CD14+ Mac, as well as from FYN+CD4+ T effector (Teff), CD8+ T, and natural killer T (NKT) to FOS+CD4+ T and RORα+ group 2 innate lymphoid cell (ILC2), along with the suppression of multiple functional immune pathways. DRD1 is implicated in regulating certain pathways and identity shifts among the hepatic immune cells. Our results provide valuable insights into the development of targeted therapies to mitigate METH-induced immune impairment. (© The Author(s) 2024. Published by Oxford University Press and Science Press on behalf of the Beijing Institute of Genomics, Chinese Academy of Sciences / China National Center for Bioinformation and Genetics Society of China.)
Databáze:	MEDLINE
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