Implications of Domestication in Theobroma cacao L. Seed-Borne Microbial Endophytes Diversity.

Autor: Toloza-Moreno DL; Centro de Investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Km 14 Vía Mosquera, Cundinamarca, Colombia., Yockteng R; Centro de Investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Km 14 Vía Mosquera, Cundinamarca, Colombia.; Institut de Systématique, Evolution, Biodiversité-UMR-CNRS 7205, Muséum National d'Histoire Naturelle, Paris, France., Pérez-Zuñiga JI; Centro de Investigación Palmira, Corporación Colombiana de Investigación Agropecuaria (AGROSAVIA), Sede Popayán, Popayán, Cauca, Colombia., Salinas-Castillo C; Departamento de Biología, Facultad de Ciencias, Max Planck Tandem Group in Holobionts, Universidad Nacional de Colombia, Bogotá, Colombia., Caro-Quintero A; Departamento de Biología, Facultad de Ciencias, Max Planck Tandem Group in Holobionts, Universidad Nacional de Colombia, Bogotá, Colombia. acaroq@unal.edu.co.
Jazyk: angličtina
Zdroj: Microbial ecology [Microb Ecol] 2024 Aug 28; Vol. 87 (1), pp. 108. Date of Electronic Publication: 2024 Aug 28.
DOI: 10.1007/s00248-024-02409-9
Abstrakt: The study of plant-microbe interactions is a rapidly growing research field, with increasing attention to the role of seed-borne microbial endophytes in protecting the plant during its development from abiotic and biotic stresses. Recent evidence suggests that seed microbiota is crucial in establishing the plant microbial community, affecting its composition and structure, and influencing plant physiology and ecology. For Theobroma cacao L., the diversity and composition of vertically transmitted microbes have yet to be addressed in detail. We explored the composition and diversity of seed-borne endophytes in cacao pods of commercial genotypes (ICS95, IMC67), recently liberated genotypes from AGROSAVIA (TCS01, TCS19), and landraces from Tumaco (Colombia) (AC9, ROS1, ROS2), to evaluate microbial vertical transmission and establishment in various tissues during plant development. We observed a higher abundance of Pseudomonas and Pantoea genera in the landraces and AGROSAVIA genotypes, while the commercial genotypes presented a higher number of bacteria species but in low abundance. In addition, all the genotypes and plant tissues showed a high percentage of fungi of the genus Penicillium. These results indicate that domestication in cacao has increased bacterial endophyte diversity but has reduced their abundance. We isolated some of these seed-borne endophytes to evaluate their potential as growth promoters and found that Bacillus, Pantoea, and Pseudomonas strains presented high production of indole acetic acid and ACC deaminase activity. Our results suggest that cacao domestication could lead to the loss of essential bacteria for seedling establishment and development. This study improves our understanding of the relationship and interaction between perennial plants and seed-borne microbiota.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: