Antiviral Effect of Microalgae Phaeodactylum tricornutum Protein Hydrolysates against Dengue Virus Serotype 2.

Autor:	Rivera-Serrano BV; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico., Cabanillas-Salcido SL; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico., Cordero-Rivera CD; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico., Jiménez-Camacho R; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico., Norzagaray-Valenzuela CD; Faculty of Biology, Autonomous University of Sinaloa, Culiacán 80019, Mexico., Calderón-Zamora L; Faculty of Biology, Autonomous University of Sinaloa, Culiacán 80019, Mexico., De Jesús-González LA; Unidad de Investigación Biomédica de Zacatecas, Instituto Mexicano del Seguro Social (IMSS), Zacatecas 98000, Mexico., Reyes-Ruiz JM; Unidad Médica de Alta Especialidad, Hospital de Especialidades No. 14, Centro Médico Nacional 'Adolfo Ruiz Cortines', Instituto Mexicano del Seguro Social (IMSS), Veracruz 91897, Mexico.; Facultad de Medicina, Región Veracruz, Universidad Veracruzana, Veracruz 91700, Mexico., Farfan-Morales CN; Departamento de Ciencias Naturales, Universidad Autónoma Metropolitana (UAM), Unidad Cuajimalpa, Ciudad de México 05348, Mexico., Romero-Utrilla A; Departamento de Anatomía Patológica, Instituto Mexicano del Seguro Social (IMSS), Culiacán 80200, Mexico., Ruíz-Ruelas VM; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico., Camberos-Barraza J; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico., Camacho-Zamora A; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico., De la Herrán-Arita AK; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico., Angulo-Rojo C; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Departamento de Anatomía Patológica, Instituto Mexicano del Seguro Social (IMSS), Culiacán 80200, Mexico., Guadrón-Llanos AM; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Maestría en Ciencias en Medicina Traslacional y Salud Publica, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico., Rábago-Monzón ÁR; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Doctorado en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico., Perales-Sánchez JXK; Bioprocesses Laboratory, Faculty of Chemical-Biological Sciences, Autonomous University of Sinaloa, Culiacán 80246, Mexico., Valdez-Flores MA; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico., Del Ángel RM; Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City 07360, Mexico., Osuna-Ramos JF; Faculty of Medicine, Autonomous University of Sinaloa, Culiacán 80246, Mexico.; Programa de Maestría en Ciencias en Biomedicina Molecular, Facultad de Medicina, Universidad Autónoma de Sinaloa (UAS), Culiacán 80246, Mexico.; Departamento de Anatomía Patológica, Instituto Mexicano del Seguro Social (IMSS), Culiacán 80200, Mexico.
Jazyk:	angličtina
Zdroj:	Marine drugs [Mar Drugs] 2024 Aug 14; Vol. 22 (8). Date of Electronic Publication: 2024 Aug 14.
DOI:	10.3390/md22080369
Abstrakt:	Dengue, caused by the dengue virus (DENV), is a global health threat transmitted by Aedes mosquitoes, resulting in 400 million cases annually. The disease ranges from mild to severe, with potential progression to hemorrhagic dengue. Current research is focused on natural antivirals due to challenges in vector control. This study evaluates the antiviral potential of peptides derived from the microalgae Phaeodactylum tricornutum , known for its bioactive compounds. Microalgae were cultivated under controlled conditions, followed by protein extraction and hydrolysis to produce four peptide fractions. These fractions were assessed for cytotoxicity via the MTT assay and antiviral activity against DENV serotype 2 using flow cytometry and plaque formation assays. The 10-30 kDa peptide fraction, at 150 and 300 μg/mL concentrations, demonstrated no cytotoxicity and significantly reduced the percentage of infected cells and viral titers. These findings suggest that peptides derived from Phaeodactylum tricornutum exhibit promising antiviral activity against dengue virus serotype 2, potentially contributing to developing new therapeutic approaches for dengue.
Databáze:	MEDLINE
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